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Literature summary for 5.6.1.2 extracted from

  • Toba, S.; Fox, L.A.; Sakakibara, H.; Porter, M.E.; Oiwa, K.; Sale, W.S.
    Distinct roles of 1alpha and 1beta heavy chains of the inner arm dynein I1 of Chlamydomonas flagella (2011), Mol. Biol. Cell, 22, 342-353.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.2

Activating Compound

Activating Compound Comment Organism Structure
microtubule the ATPase activities of the two-headed I1 dynein and the single-headed I1beta dynein are activated by microtubules, whereas the kcat of I1beta is higher than that of the intact I1d dynein Chlamydomonas sp.
additional information I1alpha does not show any microtubule activation on its ATPase activity Chlamydomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 ATP the I1alpha complex has a higher Km value than either I1 or I1beta dynein complex, indicating a lower affinity for ATP Chlamydomonas sp.
additional information
-
 additional information the steady-state ATPase rates fit the Michaelis-Menten kinetics Chlamydomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Chlamydomonas sp. 5737
-

Organism

Organism UniProt Comment Textmining
Chlamydomonas sp.
-
 several strains
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 I1beta complex has 7fold higher ATPase activity and microtubule gliding motility compared to Ialpha complex. The ATPase activity of the two-headed I1 dynein is lower than the combined value of its I1alpha and I1beta motor domains Chlamydomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n
-
 Chlamydomonas sp. ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Synonyms

Synonyms Comment Organism
dynein I1 a two-headed inner dynein arm for regulation of flagellar blending Chlamydomonas sp.
Ialpha complex dynein I1, single-headed inner dynein arm Chlamydomonas sp.
Ibeta complex dynein I1 inner dynein arm Chlamydomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 ATP I1beta has a higher turnover number compared to the two-headed wild-type I1 dynein, whereas the tunrover number of I1alpha is approximately 1/8 the one of I1beta Chlamydomonas sp.

General Information

General Information Comment Organism
physiological function a two-headed inner dynein arm for regulation of flagellar blending Chlamydomonas sp.