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Literature summary for 5.6.1.2 extracted from

  • Cho, C.; Reck-Peterson, S.L.; Vale, R.D.
    Regulatory ATPase sites of cytoplasmic dynein affect processivity and force generation (2008), J. Biol. Chem., 283, 25839-25845.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.2

Protein Variants

Protein Variants Comment Organism
E2488Q mutation in nucleotide-binding domain AAA3 Saccharomyces cerevisiae
E2819Q mutation in nucleotide-binding domain AAA4 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00003
-
 a dynein associated with a microtubule at position n mutant E2488Q Saccharomyces cerevisiae
0.00009
-
 a dynein associated with a microtubule at position n mutant E2819Q Saccharomyces cerevisiae
0.00059
-
 a dynein associated with a microtubule at position n
-
 Saccharomyces cerevisiae
0.0237
-
 ATP mutant E2488Q, microtubule-stimulated Saccharomyces cerevisiae
0.0253
-
 ATP mutant E2819Q, microtubule-stimulated Saccharomyces cerevisiae
0.0262
-
 ATP microtubule-stimulated Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + + a dynein associated with a microtubule at position n
-
 Saccharomyces cerevisiae ADP + phosphate + alpha/beta tubulin heterodimers
-
 ?
ATP + H2O + a dynein associated with a microtubule at position n microtubule-stimulated Saccharomyces cerevisiae ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Subunits

Subunits Comment Organism
More heavy chain: ca. 470000 Da Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
dynein ATPase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 ATP mutant E2488Q, basal activity Saccharomyces cerevisiae
1.38
-
 ATP mutant E2488Q, microtubule-stimulated Saccharomyces cerevisiae
3.36
-
 ATP mutant E2819Q, basal activity Saccharomyces cerevisiae
3.74
-
 ATP basal activity Saccharomyces cerevisiae
10.6
-
 ATP mutant E2819Q, microtubule-stimulated Saccharomyces cerevisiae
14.1
-
 ATP microtubule-stimulated Saccharomyces cerevisiae