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Literature summary for 5.6.1.2 extracted from

  • Johnson, K.A.
    Preparation and properties of dynein from Tetrahymena cilia (1986), Methods Enzymol., 134, 306-317.
    View publication on PubMed
Search for more literature for 5.6.1.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
 ATP 22S dynein Tetrahymena thermophila

Localization

Localization Comment Organism GeneOntology No. Textmining
cilium
-
 Tetrahymena thermophila 5929
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1900000
-
-
 Tetrahymena thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n Tetrahymena thermophila
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Organism

Organism UniProt Comment Textmining
Tetrahymena thermophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
high salt extraction, 0.6 M KCl, DEAE-Sephacel, 5-25% sucrose gradient centrifugation Tetrahymena thermophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
 22S dynein Tetrahymena thermophila

Storage Stability

Storage Stability Organism
0°C, several days Tetrahymena thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n
-
 Tetrahymena thermophila ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?