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Literature summary for 5.6.1.2 extracted from

  • King, S.M.; Otter, T.; Witman, G.B.
    Purification and characterization of Chlamydomonas flagellar dyneins (1986), Methods Enzymol., 134, 291-306.
    View publication on PubMed
Search for more literature for 5.6.1.2

Localization

Localization Comment Organism GeneOntology No. Textmining
flagellum of sperms Chlamydomonas reinhardtii
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 alpha,LC1, 1 * 440000 + 1 * 16000, 18S dynein, alpha subunit Chlamydomonas reinhardtii
18000
-
 alpha,LC1,LC2, 1 * 400000 + 1 * 22000 + 1 * 18000, 12S dynein Chlamydomonas reinhardtii
22000
-
 alpha,LC1,LC2, 1 * 400000 + 1 * 22000 + 1 * 18000, 12S dynein Chlamydomonas reinhardtii
69000
-
 alpha, IC1,IC2,LC1-7, 1 * 420000 + 1 * 78000 + 1 * 69000 + 7 * 8000-20000, 18S dynein, beta subunit Chlamydomonas reinhardtii
78000
-
 alpha, IC1,IC2,LC1-7, 1 * 420000 + 1 * 78000 + 1 * 69000 + 7 * 8000-20000, 18S dynein, beta subunit Chlamydomonas reinhardtii
400000
-
 alpha,LC1,LC2, 1 * 400000 + 1 * 22000 + 1 * 18000, 12S dynein Chlamydomonas reinhardtii
420000
-
 alpha, IC1,IC2,LC1-7, 1 * 420000 + 1 * 78000 + 1 * 69000 + 7 * 8000-20000, 18S dynein, beta subunit Chlamydomonas reinhardtii
440000
-
 alpha,LC1, 1 * 440000 + 1 * 16000, 18S dynein, alpha subunit Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n Chlamydomonas reinhardtii
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 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
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-
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Purification (Commentary)

Purification (Comment) Organism
extraction of axonemes with high salt, 0.6 M KCl, 5-20% sucrose gradient centrifugation, hydroxylapatite Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n
-
 Chlamydomonas reinhardtii ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Subunits

Subunits Comment Organism
oligomer alpha,LC1, 1 * 440000 + 1 * 16000, 18S dynein, alpha subunit Chlamydomonas reinhardtii
oligomer alpha, IC1,IC2,LC1-7, 1 * 420000 + 1 * 78000 + 1 * 69000 + 7 * 8000-20000, 18S dynein, beta subunit Chlamydomonas reinhardtii
trimer alpha,LC1,LC2, 1 * 400000 + 1 * 22000 + 1 * 18000, 12S dynein Chlamydomonas reinhardtii