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Literature summary for 5.6.1.2 extracted from

  • Pratt, M.M.
    Purification of cytoplasmic dynein from Strongylocentrotus sea urchin eggs (1986), Methods Enzymol., 134, 325-337.
    View publication on PubMed
Search for more literature for 5.6.1.2

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Strongylocentrotus purpuratus 5737
-
cytoplasm
-
 Strongylocentrotus droebachiensis 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Strongylocentrotus purpuratus
Ca2+
-
 Strongylocentrotus droebachiensis
Mg2+
-
 Strongylocentrotus purpuratus
Mg2+
-
 Strongylocentrotus droebachiensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
320000
-
 alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus purpuratus
320000
-
 alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus droebachiensis
360000
-
 alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus purpuratus
360000
-
 alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus droebachiensis
385000
-
 alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus purpuratus
385000
-
 alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus droebachiensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n Strongylocentrotus purpuratus
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
ATP + H2O + a dynein associated with a microtubule at position n Strongylocentrotus droebachiensis
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Organism

Organism UniProt Comment Textmining
Strongylocentrotus droebachiensis
-
 sea urchin
-
Strongylocentrotus purpuratus
-
 sea urchin
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sephacel, Calmodulin-affinity column, 5-20% sucrose gradient centrifugation Strongylocentrotus purpuratus
ammonium sulfate precipitation, DEAE-Sephacel, Calmodulin-affinity column, 5-20% sucrose gradient centrifugation Strongylocentrotus droebachiensis

Source Tissue

Source Tissue Comment Organism Textmining
egg
-
 Strongylocentrotus purpuratus
-
egg
-
 Strongylocentrotus droebachiensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 sucrose gradient pool Strongylocentrotus purpuratus
0.2
-
 sucrose gradient pool Strongylocentrotus droebachiensis

Storage Stability

Storage Stability Organism
0°C, buffered solution with protease inhibitors, 5 days, 20%, -80°C, liqid nitrogen, 6 months, 20% Strongylocentrotus purpuratus
0°C, buffered solution with protease inhibitors, 5 days, 20%, -80°C, liqid nitrogen, 6 months, 20% Strongylocentrotus droebachiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n
-
 Strongylocentrotus purpuratus ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?
ATP + H2O + a dynein associated with a microtubule at position n
-
 Strongylocentrotus droebachiensis ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Subunits

Subunits Comment Organism
oligomer alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus purpuratus
oligomer alpha,beta,gamma, 1 * 385000 + 1 * 360000 + 1 * 320000, only heavy chains determined, alpha and gamma band slit into doublets, several unidentified lower molecular weight polypeptides Strongylocentrotus droebachiensis