Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | from porcine brain, 3-to 8fold | Hemicentrotus pulcherrimus | |
sea urchin egg | 3-to 8fold | Hemicentrotus pulcherrimus | |
Triton X-100 | 3.5fold activation, 0.1% | Hemicentrotus pulcherrimus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
erythro-9-[3-(2-hydroxynonyl)]adenine | half maximal inhibition at 3 to 5 mM | Hemicentrotus pulcherrimus | |
VO43- | 50% inhibition at 0.001 mM | Hemicentrotus pulcherrimus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Hemicentrotus pulcherrimus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
550000 | - |
12S sucrose density gradient fraction, calculated from sedimentation constant (12.3 S) and Stokes radius (10.2 nm) | Hemicentrotus pulcherrimus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a dynein associated with a microtubule at position n | Hemicentrotus pulcherrimus | - |
ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end) | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hemicentrotus pulcherrimus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Sepharose 4B, hydroxyapatite column, 5-20% sucrose density gradient | Hemicentrotus pulcherrimus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg | - |
Hemicentrotus pulcherrimus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.8 | - |
12S sucrose gradient fraction | Hemicentrotus pulcherrimus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + a dynein associated with a microtubule at position n | - |
Hemicentrotus pulcherrimus | ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end) | - |
? |