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Literature summary for 5.6.1.2 extracted from

  • Belles-Isles, M.; Chapeau, C.; White, D.; Gagnon, C.
    Isolation and characterization of dynein ATPase from bull spermatozoa (1986), Biochem. J., 240, 863-869.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.2

Inhibitors

Inhibitors Comment Organism Structure
erythro-9-[3-(2-hydroxynonyl)]adenine 1 mM, 48% inhibition Bos taurus
VO43-
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
43
-
 ATP 19S sucrose gradient dynein particle Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ preference in decreasing order: Mn2+, Mg2+, Ca2+, Ni2+ Bos taurus
Mg2+ chelation of Mg2+ with EDTA completely blocks ATP hydrolysis Bos taurus
Mn2+ preference in decreasing order: Mn2+, Mg2+, Ca2+, Ni2+ Bos taurus
Ni2+ preference in decreasing order: Mn2+, Mg2+, Ca2+, Ni2+ Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n Bos taurus
-
 ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
low salt extraction, 0.1% Triton X-100, 0.1 mM PMSF, 200 mM sucrose 25 mM potassium glutamate, 1 mM dithiothreitol and 35 mM Tris-HCl, pH 7.9, 5-25% sucrose gradient Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
 19S sucrose gradient dynein particle, substrate GTP Bos taurus
0.07
-
 low salt extract Bos taurus
0.09
-
 19S sucrose gradient dynein particle, substrate ITP Bos taurus
0.11
-
 19S sucrose gradient dynein particle, substrate UTP Bos taurus
0.29
-
 19S sucrose gradient dynein particle, substrate CTP Bos taurus
0.3
-
 12S sucrose gradient dynein particle Bos taurus
1.2
-
 19S sucrose gradient dynein particle Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n
-
 Bos taurus ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
 ?

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 linear increase from pH 6 to pH 9, sharp peak at pH 9.5 Bos taurus