Literature summary for 5.6.1.2 extracted from

Yokota, E.; Mabuchi, I.; Sato, H.
Activation of sea urchin sperm flagellar dynein ATPase activity by salt-extracted axonemes (1987), J. Biochem., 102, 31-41.

Search for more literature for 5.6.1.2

Activating Compound

Activating Compound	Comment	Organism	Structure
axonemes	salt-extracted axonemes cause a 7 to 8fold activation of 21S dynein, maximum activation with axoneme/dynein weight ratio 14/1, low ionic strength, pH 7.9-8.3	Pseudocentrotus depressus
KCl	linear increase in the range of 0.1 M and 0.6 M KCl	Pseudocentrotus depressus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + a dynein associated with a microtubule at position n	Pseudocentrotus depressus	-	ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudocentrotus depressus	-	sea urchin	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.05	-	21S sucrose gradient fraction, 4 mM MgSO4, 0.5 mM EDTA, 1 mM ATP, pH 8	Pseudocentrotus depressus
0.08	-	21S sucrose gradient fraction, 4 mM MgSO4, 0.5 mM EDTA, 1 mM ATP, pH 8, in the absence of KCL	Pseudocentrotus depressus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + a dynein associated with a microtubule at position n	-	Pseudocentrotus depressus	ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.2	-	-	Pseudocentrotus depressus

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.7	8.2	-	Pseudocentrotus depressus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)