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Literature summary for 5.6.1.2 extracted from
- Inner arm dynein ATPase fraction of sea urchin sperm flagella causes active sliding of axonemal outer doublet microtubule (1991), Biochem. Biophys. Res. Commun., 175, 173-178.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
ATP | 12S sucrose gradient fraction of inner arm dynein | Hemicentrotus pulcherrimus |  |
| 12 | - |
ATP | 12S sucrose gradient fraction of inner arm dynein | Heliocidaris crassispina | |
| 46 | - |
ATP | 21S sucrose gradient fraction of outer arm dynein | Hemicentrotus pulcherrimus | |
| 46 | - |
ATP | 21S sucrose gradient fraction of outer arm dynein | Heliocidaris crassispina | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| flagellum | of sperms | Hemicentrotus pulcherrimus | - |
- |
| flagellum | of sperms | Heliocidaris crassispina | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a dynein associated with a microtubule at position n | Hemicentrotus pulcherrimus | - |
ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
| ATP + H2O + a dynein associated with a microtubule at position n | Heliocidaris crassispina | - |
ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Heliocidaris crassispina | - |
sea urchin | - |
| Hemicentrotus pulcherrimus | - |
sea urchin | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| high-salt extraction | Hemicentrotus pulcherrimus |
| high-salt extraction | Heliocidaris crassispina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| semen | - |
Hemicentrotus pulcherrimus | - |
| semen | - |
Heliocidaris crassispina | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.12 | - |
12S sucrose gradient fraction of inner arm dynein | Hemicentrotus pulcherrimus |
| 0.12 | - |
12S sucrose gradient fraction of inner arm dynein | Heliocidaris crassispina |
| 1.1 | - |
21S sucrose gradient fraction of outer arm dynein | Hemicentrotus pulcherrimus |
| 1.1 | - |
21S sucrose gradient fraction of outer arm dynein | Heliocidaris crassispina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a dynein associated with a microtubule at position n | - |
Hemicentrotus pulcherrimus | ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
| ATP + H2O + a dynein associated with a microtubule at position n | - |
Heliocidaris crassispina | ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end) | - |
? | |
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Release 2023.1 (January 2023)
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