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Literature summary for 5.6.1.2 extracted from

  • Kumar, S.; Lee, I.H.; Plamann, M.
    Two approaches to isolate cytoplasmic dynein ATPase from Neurospora crassa (2000), Biochimie, 82, 229-236.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
microtubules
-
Neurospora crassa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Neurospora crassa 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1500000
-
gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n Neurospora crassa
-
ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
sucrose density gradient fractionation, gel filtration Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a dynein associated with a microtubule at position n
-
Neurospora crassa ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
?
ATP + H2O + a dynein associated with a microtubule at position n hydrolysis stimulated 2fold by microtubules at an ATP concentration of 4 mM Neurospora crassa ADP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
?
CTP + H2O + a dynein associated with a microtubule at position n 44% activity compared with ATP Neurospora crassa CDP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
?
GTP + H2O + a dynein associated with a microtubule at position n 86% activity compared with ATP Neurospora crassa GDP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
?
TTP + H2O + a dynein associated with a microtubule at position n 37% activity compared with ATP Neurospora crassa TDP + phosphate + a dynein associated with a microtubule at position n-1 (toward the minus end)
-
?