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Literature summary for 5.6.1.1 extracted from

  • Fan, X.; Lin, Z.; Fan, G.; Lu, J.; Hou, Y.; Habai, G.; Sun, L.; Yu, P.; Shen, Y.; Wen, M.; Wang, C.
    The AAA protein spastin possesses two levels of basal ATPase activity (2018), FEBS Lett., 592, 1625-1633 .
    View publication on PubMed
Search for more literature for 5.6.1.1

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of spastin are grown using the vapor diffusion method at 20°C Homo sapiens

Protein Variants

Protein Variants Comment Organism
E356A the basal ATPase activity is severely compromised to undetectable levels Homo sapiens
E442Q neutralization of the positive charges in the microtubule-binding domain (MTBD) of spastin, resulting in the spastin-allA mutant, dramatically decreases the ATPase activity at low concentration, although the ATP-hydrolyzing potential is not affected. The ATP-hydrolyzing activity of spastin-allA is stimulated to varying levels by the ATPase-deficient E442Q mutant, depending on whether the microtubule-binding domain is intact Homo sapiens
G370R the basal ATPase activity is severely compromised to about 30% Homo sapiens
R562Q the basal ATPase activity is severely compromised to undetectable levels Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
spastin the basal ATPase activity of spastin is negatively regulated by spastin concentration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + microtubule Homo sapiens the enzyme plays important roles in various cellular events including axon regeneration, cytokinesis, and nuclear envelope sealing after mitosis ADP + phosphate + alpha/beta tubulin heterodimers
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UBP0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + microtubule the enzyme plays important roles in various cellular events including axon regeneration, cytokinesis, and nuclear envelope sealing after mitosis Homo sapiens ADP + phosphate + alpha/beta tubulin heterodimers
-
 ?
ATP + H2O + microtubule in addition to the AAA domain, the microtubule-binding domain region of spastin is also involved in regulating ATPase activity Homo sapiens ADP + phosphate + alpha/beta tubulin heterodimers
-
 ?

Synonyms

Synonyms Comment Organism
spastin
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme plays important roles in various cellular events including axon regeneration, cytokinesis, and nuclear envelope sealing after mitosis Homo sapiens