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Literature summary for 5.5.1.B7 extracted from

  • Pelot, K.A.; Hagelthorn, D.M.; Hong, Y.J.; Tantillo, D.J.; Zerbe, P.
    Diterpene synthase-catalyzed biosynthesis of distinct clerodane stereoisomers (2020), Chembiochem, 20, 111-117.
    View publication on PubMed
Search for more literature for 5.5.1.B7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Panicum virgatum

Protein Variants

Protein Variants Comment Organism
F251A the mutant produces (5S,8S,9R,10R)-13Z-clerodienyl diphosphate from (Z,Z,Z)-nerylneryl diphosphate with a 3fold increased activity compared to the wild type enzyme. The enzyme also produces cis,cis-clerodienyl diphosphate besides the natural product cis,trans-clerodienyl diphosphate from (E,E,E)-geranylgeranyl diphosphate Panicum virgatum
F251G the mutant shows reduced activity towards (E,E,E)-geranylgeranyl diphosphate compared to the wild type enzyme Panicum virgatum
F251S the mutant shows reduced activity towards (E,E,E)-geranylgeranyl diphosphate compared to the wild type enzyme Panicum virgatum
F251V the mutant produces (5S,8S,9R,10R)-13Z-clerodienyl diphosphate from (Z,Z,Z)-nerylneryl diphosphate with an 8fold increased activity compared to the wild type enzyme. The enzyme also produces cis,cis-clerodienyl diphosphate besides the natural product cis,trans-clerodienyl diphosphate from (E,E,E)-geranylgeranyl diphosphate Panicum virgatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E,E,E)-geranylgeranyl diphosphate Panicum virgatum
-
 cis,trans-clerodienyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Panicum virgatum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E,E)-geranylgeranyl diphosphate
-
 Panicum virgatum cis,trans-clerodienyl diphosphate
-
 ?
(Z,Z,Z)-nerylneryl diphosphate
-
 Panicum virgatum (5S,8S,9R,10R)-13Z-clerodienyl diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
CPS1
-
 Panicum virgatum