Cloned (Comment) | Organism |
---|---|
expressed as a Flag-tagged fusion protein | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D260E | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 92%, wild-type: 100%. Relative activity: 135% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type. Mutant shows 6% activity with 24-methylene-24-dihydro-cycloartenol (wild-type 0%) | Arabidopsis thaliana |
D260H | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 61%, wild-type: 100%. Relative activity: 5.2% (wild-type: 100%). Km (cycloeucalenol) decreased compared to wild-type | Arabidopsis thaliana |
D260V | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 0%, wild-type: 100%. Relative activity: below 1.5% (wild-type: 100%) | Arabidopsis thaliana |
D260Y | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 22%, wild-type: 100%. Relative activity: 0.7% (wild-type: 100%) | Arabidopsis thaliana |
E29D | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 65%, wild-type: 100%. Relative activity: 3.8% (wild-type: 100%). Km (cycloeucalenol) decreased compared to wild-type | Arabidopsis thaliana |
E29H | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 33%, wild-type: 100%. Relative activity: 3.5% (wild-type: 100%). Km (cycloeucalenol) decreased compared to wild-type | Arabidopsis thaliana |
E29K | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 1.7%, wild-type: 100%. Relative activity: below 0.5% (wild-type: 100%) | Arabidopsis thaliana |
E29K | in vivo conversion of cycloeucalenol into ergosterol and in vitro CPI activity in Saccharomyces cerevisiae erg7 transformants carrying Arabidopsis thaliana mutant CPI: 1.7% relative ergosterol synthesis (wild-type: 100%), below 0.5% in vitro microsomal CPI activity (wild-type: 100%) | Arabidopsis thaliana |
E29V | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 0%, wild-type: 100%. Relative activity: below 0.5% (wild-type: 100%) | Arabidopsis thaliana |
E54V | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 94%, wild-type: 100% | Arabidopsis thaliana |
E58V | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 91%, wild-type: 100% | Arabidopsis thaliana |
E60V | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 83%, wild-type: 100% | Arabidopsis thaliana |
F111L | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 88%, wild-type: 100%. Relative activity: 31% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type | Arabidopsis thaliana |
F116L | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 37%, wild-type: 100%. Relative activity: 4.7% (wild-type: 100%) | Arabidopsis thaliana |
F117L | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 89%, wild-type: 100%. Relative activity: 28% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type | Arabidopsis thaliana |
G108A | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 92%, wild-type: 100%. Relative activity: 90% (wild-type: 100%) | Arabidopsis thaliana |
G108E | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 0%, wild-type: 100%. Relative activity: below 0.5% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type | Arabidopsis thaliana |
G108E | in vivo conversion of cycloeucalenol into ergosterol and in vitro CPI activity in Saccharomyces cerevisiae erg7 transformants carrying Arabidopsis thaliana mutant CPI: 0% relative ergosterol synthesis (wild-type: 100%), below 0.5% in vitro microsomal CPI activity (wild-type: 100%) | Arabidopsis thaliana |
G108V | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 6%, wild-type: 100%. Relative activity: 1.7% (wild-type: 100%) | Arabidopsis thaliana |
G28E | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 15%, wild-type: 100%. Relative activity: 1% (wild-type: 100%) | Arabidopsis thaliana |
G28E | in vivo conversion of cycloeucalenol into ergosterol and in vitro CPI activity in Saccharomyces cerevisiae erg7 transformants carrying Arabidopsis thaliana mutant CPI: 15% relative ergosterol synthesis (wild-type: 100%), 1% in vitro microsomal CPI activity (wild-type: 100%) | Arabidopsis thaliana |
G28V | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 46%, wild-type: 100%. Relative activity: 1.6% (wild-type: 100%) | Arabidopsis thaliana |
H114L | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 85%, wild-type: 100%. Relative activity: 5.2% (wild-type: 100%) | Arabidopsis thaliana |
N109A | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 76%, wild-type: 100%. Relative activity: 4.6% (wild-type: 100%) | Arabidopsis thaliana |
T113A | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 69%, wild-type: 100%. Relative activity: 7.2% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type | Arabidopsis thaliana |
T113I | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 66%, wild-type: 100%. Relative activity: 3.1% (wild-type: 100%) | Arabidopsis thaliana |
T113I | in vivo conversion of cycloeucalenol into ergosterol and in vitro CPI activity in Saccharomyces cerevisiae erg7 transformants carrying Arabidopsis thaliana mutant CPI: 66% relative ergosterol synthesis (wild-type: 100%), below 2.4% in vitro microsomal CPI activity (wild-type: 100%) | Arabidopsis thaliana |
T113S | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 98%, wild-type: 100%. Relative activity: 73% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type | Arabidopsis thaliana |
W112E | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 77%, wild-type: 100%. Relative activity: 5.8% (wild-type: 100%) | Arabidopsis thaliana |
W112K | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 47%, wild-type: 100%. Relative activity: 1.8% (wild-type: 100%) | Arabidopsis thaliana |
W112L | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 86%, wild-type: 100%. Relative activity: 49% (wild-type: 100%). Km (cycloeucalenol) decreased compared to wild-type. Mutant shows 69% activity with 24-methylene-24-dihydro-cycloartenol (wild-type 0%), 10% activity with cycloartenol (wild-type 0%). 32% activity with cycloartanol (wild-type 0%), 15% activity with 4beta-methyl-24-dihydro-cycloeucalenol (wild-type 0%) | Arabidopsis thaliana |
Y110L | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 64%, wild-type: 100%. Relative activity: 2.8% (wild-type: 100%) | Arabidopsis thaliana |
Y115L | cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 83%, wild-type: 100%. Relative activity: 4.3% (wild-type: 100%) | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.077 | - |
cycloeucalenol | mutant E29D, 30°C, pH not specified in the publication, Vmax: 0.24 nmol/h/mg | Arabidopsis thaliana | |
0.078 | - |
cycloeucalenol | mutant D260H, 30°C, pH not specified in the publication, Vmax: 0.35 nmol/h/mg | Arabidopsis thaliana | |
0.093 | - |
cycloeucalenol | mutant E29H, 30°C, pH not specified in the publication, Vmax: 0.33 nmol/h/mg | Arabidopsis thaliana | |
0.135 | - |
cycloeucalenol | mutant W112L, 30°C, pH not specified in the publication, Vmax: 14.7 nmol/h/mg | Arabidopsis thaliana | |
0.167 | - |
cycloeucalenol | wild-type, 30°C, pH not specified in the publication, Vmax: 22 nmol/h/mg | Arabidopsis thaliana | |
0.377 | - |
cycloeucalenol | mutant F111L, 30°C, pH not specified in the publication, Vmax: 4.7 nmol/h/mg | Arabidopsis thaliana | |
0.409 | - |
cycloeucalenol | mutant D260E, 30°C, pH not specified in the publication, Vmax: 44 nmol/h/mg | Arabidopsis thaliana | |
0.496 | - |
cycloeucalenol | mutant T113S, 30°C, pH not specified in the publication, Vmax: 26 nmol/h/mg | Arabidopsis thaliana | |
0.52 | - |
cycloeucalenol | mutant F117L, 30°C, pH not specified in the publication, Vmax: 5.3 nmol/h/mg | Arabidopsis thaliana | |
0.622 | - |
cycloeucalenol | mutant G108A, 30°C, pH not specified in the publication, Vmax: 11.4 nmol/h/mg | Arabidopsis thaliana | |
1.53 | - |
cycloeucalenol | mutant T113A, 30°C, pH not specified in the publication, Vmax: 4.9 nmol/h/mg | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cycloeucalenol | - |
Arabidopsis thaliana | obtusifoliol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Atcpi | - |
Arabidopsis thaliana |