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Literature summary for 5.5.1.7 extracted from

  • Moiseeva, O.V.; Belova, O.V.; Solyanikova, I.P.; Schlomann, M.; Golovleva, L.A.
    Enzymes of a new modified ortho-pathway utilizing 2-chlorophenol in Rhodococcus opacus 1CP (2001), Biochemistry (Moscow), 66, 548-555.
    View publication on PubMed
Search for more literature for 5.5.1.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1429
-
 2-chloro-cis,cis-muconate
-
 Rhodococcus opacus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rhodococcus opacus key enzyme in a modified ortho-pathway in cells utilizing 2-chlorophenol via a 3-chlorocatechol branch of a modified ortho-pathway ?
-
 ?
additional information Rhodococcus opacus 1CP key enzyme in a modified ortho-pathway in cells utilizing 2-chlorophenol via a 3-chlorocatechol branch of a modified ortho-pathway ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus opacus
-
 strain1CP
-
Rhodococcus opacus 1CP
-
 strain1CP
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus opacus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18
-
-
 Rhodococcus opacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Chloro-cis,cis-muconate
-
 Rhodococcus opacus 5-Chloromuconolactone
-
 ?
2-Chloro-cis,cis-muconate
-
 Rhodococcus opacus 1CP 5-Chloromuconolactone
-
 ?
additional information no activity with cis,cis-muconate, 3-chloromuconate, 3-methylmuconate and 2,4-dichloromuconate Rhodococcus opacus ?
-
 ?
additional information key enzyme in a modified ortho-pathway in cells utilizing 2-chlorophenol via a 3-chlorocatechol branch of a modified ortho-pathway Rhodococcus opacus ?
-
 ?
additional information no activity with cis,cis-muconate, 3-chloromuconate, 3-methylmuconate and 2,4-dichloromuconate Rhodococcus opacus 1CP ?
-
 ?
additional information key enzyme in a modified ortho-pathway in cells utilizing 2-chlorophenol via a 3-chlorocatechol branch of a modified ortho-pathway Rhodococcus opacus 1CP ?
-
 ?

Synonyms

Synonyms Comment Organism
CMCI
-
 Rhodococcus opacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Rhodococcus opacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
171000
-
 2-chloro-cis,cis-muconate
-
 Rhodococcus opacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7
-
 in borate-phosphate-acetate buffer Rhodococcus opacus
6.2
-
 in bis-Tris-HCl and Tris-HCl buffer Rhodococcus opacus