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Literature summary for 5.5.1.6 extracted from

  • Park, S.H.; Lee, C.W.; Cho, S.M.; Lee, H.; Park, H.; Lee, J.; Lee, J.H.
    Crystal structure and enzymatic properties of chalcone isomerase from the Antarctic vascular plant Deschampsia antarctica Desv (2018), PLoS ONE, 13, e0192415 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.5.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Deschampsia antarctica

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme and S189A mutant incomplex with isoliquiritigenin, sitting drop vapor diffusion method, using 0.2Mammonium sulfate, 0.1MMES (pH 7.3), and 29% (w/v) PEG 5000 MME Deschampsia antarctica

Protein Variants

Protein Variants Comment Organism
I190M the mutant shows increased activity compared to the wild type enzyme Deschampsia antarctica
K108A the mutant shows reduced activity compared to the wild type enzyme Deschampsia antarctica
M36A the mutant shows reduced activity compared to the wild type enzyme Deschampsia antarctica
M96K the mutant shows reduced activity compared to the wild type enzyme Deschampsia antarctica
Q104E the mutant shows increased activity compared to the wild type enzyme Deschampsia antarctica
S189A the mutant shows reduced activity compared to the wild type enzyme Deschampsia antarctica
S189T the mutant shows increased activity compared to the wild type enzyme Deschampsia antarctica
Y116A the mutant shows reduced activity compared to the wild type enzyme Deschampsia antarctica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
 naringenin chalcone mutant enzyme S189A, at pH 7.6 and 25°C Deschampsia antarctica
0.0048
-
 naringenin chalcone mutant enzyme K108A, at pH 7.6 and 25°C Deschampsia antarctica
0.0048
-
 naringenin chalcone mutant enzyme M36A, at pH 7.6 and 25°C Deschampsia antarctica
0.0053
-
 isoliquiritigenin mutant enzyme R34A, at pH 7.6 and 25°C Deschampsia antarctica
0.0061
-
 naringenin chalcone mutant enzyme Q104E, at pH 7.6 and 25°C Deschampsia antarctica
0.007
-
 naringenin chalcone mutant enzyme Y116A, at pH 7.6 and 25°C Deschampsia antarctica
0.008
-
 isoliquiritigenin mutant enzyme I190M, at pH 7.6 and 25°C Deschampsia antarctica
0.0085
-
 naringenin chalcone wild type enzyme, at pH 7.6 and 25°C Deschampsia antarctica
0.0086
-
 naringenin chalcone mutant enzyme I190M, at pH 7.6 and 25°C Deschampsia antarctica
0.0099
-
 naringenin chalcone mutant enzyme S189T, at pH 7.6 and 25°C Deschampsia antarctica
0.0105
-
 naringenin chalcone mutant enzyme M96K, at pH 7.6 and 25°C Deschampsia antarctica
0.0108
-
 isoliquiritigenin mutant enzyme S189T, at pH 7.6 and 25°C Deschampsia antarctica
0.0112
-
 isoliquiritigenin mutant enzyme K108A, at pH 7.6 and 25°C Deschampsia antarctica
0.0153
-
 isoliquiritigenin wild type enzyme, at pH 7.6 and 25°C Deschampsia antarctica
0.0164
-
 isoliquiritigenin mutant enzyme M36A, at pH 7.6 and 25°C Deschampsia antarctica
0.0185
-
 isoliquiritigenin mutant enzyme S189A, at pH 7.6 and 25°C Deschampsia antarctica
0.0225
-
 isoliquiritigenin mutant enzyme Q104E, at pH 7.6 and 25°C Deschampsia antarctica
0.0243
-
 isoliquiritigenin mutant enzyme R34M, at pH 7.6 and 25°C Deschampsia antarctica
0.0276
-
 isoliquiritigenin mutant enzyme Y116A, at pH 7.6 and 25°C Deschampsia antarctica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a chalcone Deschampsia antarctica
-
 a flavanone
-
 ?

Organism

Organism UniProt Comment Textmining
Deschampsia antarctica G4U3G8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Deschampsia antarctica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a chalcone
-
 Deschampsia antarctica a flavanone
-
 ?
isoliquiritigenin low activity compared to naringenin chalcone Deschampsia antarctica liquiritigenin
-
 ?
naringenin chalcone preferred substrate Deschampsia antarctica naringenin
-
 ?

Synonyms

Synonyms Comment Organism
Chi1
-
 Deschampsia antarctica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Deschampsia antarctica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 70 the enzymatic activity rapidly decreases at temperatures over 60°C, and was almost completely lost at 70°C. The enzyme enzyme shows greater than 50% activity after 30 min of preincubation at 40°C, whereas the activity is completely lost after pre-incubation at 60°C Deschampsia antarctica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 isoliquiritigenin mutant enzyme R34A, at pH 7.6 and 25°C Deschampsia antarctica
0.002
-
 isoliquiritigenin mutant enzyme R34M, at pH 7.6 and 25°C Deschampsia antarctica
0.003
-
 isoliquiritigenin mutant enzyme K108A, at pH 7.6 and 25°C Deschampsia antarctica
0.003
-
 isoliquiritigenin mutant enzyme S189A, at pH 7.6 and 25°C Deschampsia antarctica
0.005
-
 isoliquiritigenin mutant enzyme I190M, at pH 7.6 and 25°C Deschampsia antarctica
0.007
-
 isoliquiritigenin mutant enzyme Y116A, at pH 7.6 and 25°C Deschampsia antarctica
0.008
-
 isoliquiritigenin mutant enzyme M36A, at pH 7.6 and 25°C Deschampsia antarctica
0.017
-
 isoliquiritigenin mutant enzyme S189T, at pH 7.6 and 25°C Deschampsia antarctica
0.02
-
 isoliquiritigenin wild type enzyme, at pH 7.6 and 25°C Deschampsia antarctica
0.023
-
 isoliquiritigenin mutant enzyme Q104E, at pH 7.6 and 25°C Deschampsia antarctica
5
-
 naringenin chalcone mutant enzyme S189A, at pH 7.6 and 25°C Deschampsia antarctica
7.8
-
 naringenin chalcone mutant enzyme M96K, at pH 7.6 and 25°C Deschampsia antarctica
9.6
-
 naringenin chalcone mutant enzyme Y116A, at pH 7.6 and 25°C Deschampsia antarctica
35.2
-
 naringenin chalcone mutant enzyme M36A, at pH 7.6 and 25°C Deschampsia antarctica
56.9
-
 naringenin chalcone mutant enzyme K108A, at pH 7.6 and 25°C Deschampsia antarctica
130.3
-
 naringenin chalcone wild type enzyme, at pH 7.6 and 25°C Deschampsia antarctica
134.7
-
 naringenin chalcone mutant enzyme Q104E, at pH 7.6 and 25°C Deschampsia antarctica
197.7
-
 naringenin chalcone mutant enzyme I190M, at pH 7.6 and 25°C Deschampsia antarctica
228.2
-
 naringenin chalcone mutant enzyme S189T, at pH 7.6 and 25°C Deschampsia antarctica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Deschampsia antarctica

pH Range

pH Minimum pH Maximum Comment Organism
7 10 about 55% activity at pH 7.0, 100% activity at pH around 8.0, about 80% activity at pH 9.0, about 40% activity at pH 10.0 Deschampsia antarctica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.069
-
 isoliquiritigenin mutant enzyme R34M, at pH 7.6 and 25°C Deschampsia antarctica
0.18
-
 isoliquiritigenin mutant enzyme S189A, at pH 7.6 and 25°C Deschampsia antarctica
0.242
-
 isoliquiritigenin mutant enzyme Y116A, at pH 7.6 and 25°C Deschampsia antarctica
0.298
-
 isoliquiritigenin mutant enzyme K108A, at pH 7.6 and 25°C Deschampsia antarctica
0.314
-
 isoliquiritigenin mutant enzyme R34A, at pH 7.6 and 25°C Deschampsia antarctica
0.508
-
 isoliquiritigenin mutant enzyme M36A, at pH 7.6 and 25°C Deschampsia antarctica
0.625
-
 isoliquiritigenin mutant enzyme I190M, at pH 7.6 and 25°C Deschampsia antarctica
1.011
-
 isoliquiritigenin mutant enzyme Q104E, at pH 7.6 and 25°C Deschampsia antarctica
1.416
-
 isoliquiritigenin wild type enzyme, at pH 7.6 and 25°C Deschampsia antarctica
1.543
-
 isoliquiritigenin mutant enzyme S189T, at pH 7.6 and 25°C Deschampsia antarctica
743
-
 naringenin chalcone mutant enzyme M96K, at pH 7.6 and 25°C Deschampsia antarctica
1380
-
 naringenin chalcone mutant enzyme Y116A, at pH 7.6 and 25°C Deschampsia antarctica
2080
-
 naringenin chalcone mutant enzyme S189A, at pH 7.6 and 25°C Deschampsia antarctica
7330
-
 naringenin chalcone mutant enzyme M36A, at pH 7.6 and 25°C Deschampsia antarctica
11900
-
 naringenin chalcone mutant enzyme K108A, at pH 7.6 and 25°C Deschampsia antarctica
15300
-
 naringenin chalcone wild type enzyme, at pH 7.6 and 25°C Deschampsia antarctica
22080
-
 naringenin chalcone mutant enzyme Q104E, at pH 7.6 and 25°C Deschampsia antarctica
22920
-
 naringenin chalcone mutant enzyme I190M, at pH 7.6 and 25°C Deschampsia antarctica
23060
-
 naringenin chalcone mutant enzyme S189T, at pH 7.6 and 25°C Deschampsia antarctica