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Literature summary for 5.5.1.6 extracted from

  • Cheng, A.X.; Zhang, X.; Han, X.J.; Zhang, Y.Y.; Gao, S.; Liu, C.J.; Lou, H.X.
    Identification of chalcone isomerase in the basal land plants reveals an ancient evolution of enzymatic cyclization activity for synthesis of flavonoids (2018), New Phytol., 217, 909-924 .
    View publication on PubMed
Search for more literature for 5.5.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells and in the Arabidopsis thaliana tt5 mutant Medicago sativa
expressed in Escherichia coli BL21 cells and in the Arabidopsis thaliana tt5 mutant Marchantia paleacea
expressed in Escherichia coli BL21 cells and in the Arabidopsis thaliana tt5 mutant Selaginella moellendorffii
expressed in Escherichia coli BL21 cells and in the Arabidopsis thaliana tt5 mutant Physcomitrella appendiculatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02987
-
 6'-Deoxychalcone at pH 7.5 and 35°C Medicago sativa
0.0349
-
 6'-Deoxychalcone at pH 7.5 and 35°C Marchantia paleacea
0.0532
-
 6'-Deoxychalcone at pH 7.5 and 35°C Physcomitrella appendiculatum
0.0535
-
 6'-hydroxychalcone at pH 7.5 and 35°C Marchantia paleacea
0.05365
-
 6'-hydroxychalcone at pH 7.5 and 35°C Medicago sativa
0.05817
-
 6'-Deoxychalcone at pH 7.5 and 35°C Selaginella moellendorffii
0.07752
-
 6'-hydroxychalcone at pH 7.5 and 35°C Physcomitrella appendiculatum
0.08478
-
 6'-hydroxychalcone at pH 7.5 and 35°C Selaginella moellendorffii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Medicago sativa 5737
-
cytoplasm
-
 Marchantia paleacea 5737
-
cytoplasm
-
 Selaginella moellendorffii 5737
-
cytoplasm
-
 Physcomitrella appendiculatum 5737
-
nucleus
-
 Medicago sativa 5634
-
nucleus
-
 Marchantia paleacea 5634
-
nucleus
-
 Selaginella moellendorffii 5634
-
nucleus
-
 Physcomitrella appendiculatum 5634
-

Organism

Organism UniProt Comment Textmining
Marchantia paleacea
-
-
-
Medicago sativa
-
-
-
Physcomitrella appendiculatum
-
-
-
Selaginella moellendorffii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6'-deoxychalcone
-
 Medicago sativa (2S)-liquiritigenin
-
 ?
6'-deoxychalcone
-
 Marchantia paleacea (2S)-liquiritigenin
-
 ?
6'-deoxychalcone
-
 Selaginella moellendorffii (2S)-liquiritigenin
-
 ?
6'-deoxychalcone
-
 Physcomitrella appendiculatum (2S)-liquiritigenin
-
 ?
6'-hydroxychalcone
-
 Medicago sativa (2S)-naringenin
-
 ?
6'-hydroxychalcone
-
 Marchantia paleacea (2S)-naringenin
-
 ?
6'-hydroxychalcone
-
 Selaginella moellendorffii (2S)-naringenin
-
 ?
6'-hydroxychalcone
-
 Physcomitrella appendiculatum (2S)-naringenin
-
 ?

Synonyms

Synonyms Comment Organism
CHI
-
 Medicago sativa
CHI
-
 Marchantia paleacea
CHI
-
 Selaginella moellendorffii
CHI
-
 Physcomitrella appendiculatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.44
-
 6'-Deoxychalcone at pH 7.5 and 35°C Selaginella moellendorffii
0.76
-
 6'-Deoxychalcone at pH 7.5 and 35°C Physcomitrella appendiculatum
7.06
-
 6'-Deoxychalcone at pH 7.5 and 35°C Medicago sativa
7.3
-
 6'-Deoxychalcone at pH 7.5 and 35°C Marchantia paleacea
70.4
-
 6'-hydroxychalcone at pH 7.5 and 35°C Selaginella moellendorffii
75.6
-
 6'-hydroxychalcone at pH 7.5 and 35°C Physcomitrella appendiculatum
103
-
 6'-hydroxychalcone at pH 7.5 and 35°C Medicago sativa
108.7
-
 6'-hydroxychalcone at pH 7.5 and 35°C Marchantia paleacea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.5
-
 6'-Deoxychalcone at pH 7.5 and 35°C Selaginella moellendorffii
21.5
-
 6'-Deoxychalcone at pH 7.5 and 35°C Physcomitrella appendiculatum
236.7
-
 6'-Deoxychalcone at pH 7.5 and 35°C Medicago sativa
236.7
-
 6'-Deoxychalcone at pH 7.5 and 35°C Marchantia paleacea
830
-
 6'-hydroxychalcone at pH 7.5 and 35°C Selaginella moellendorffii
975
-
 6'-hydroxychalcone at pH 7.5 and 35°C Physcomitrella appendiculatum
1916.7
-
 6'-hydroxychalcone at pH 7.5 and 35°C Medicago sativa
1916.7
-
 6'-hydroxychalcone at pH 7.5 and 35°C Marchantia paleacea