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Literature summary for 5.5.1.6 extracted from

  • Thomsen, M.; Tuukkanen, A.; Dickerhoff, J.; Palm, G.J.; Kratzat, H.; Svergun, D.I.; Weisz, K.; Bornscheuer, U.T.; Hinrichs, W.
    Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase (2015), Acta Crystallogr. Sect. D, 71, 907-917 .
    View publication on PubMed
Search for more literature for 5.5.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Eubacterium ramulus

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with (2S)-naringenin, hanging drop vapor diffusion method, using 2.0 M ammonium sulfate, 0.2 M NaCl, 0.1 M HEPES pH 7.5, 6% (v/v) ethanol, 0.37 mM naringenin chalcone Eubacterium ramulus

Protein Variants

Protein Variants Comment Organism
H33A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Eubacterium ramulus
H33E the mutant shows reduced catalytic efficiency compared to the wild type enzyme Eubacterium ramulus
H33Q the mutant shows reduced catalytic efficiency compared to the wild type enzyme Eubacterium ramulus
H73A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Eubacterium ramulus
R125A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Eubacterium ramulus
Y48F the mutant shows increased catalytic efficiency compared to the wild type enzyme Eubacterium ramulus

General Stability

General Stability Organism
the activity is not affected by DMSO concentrations of up to 5% (v/v) Eubacterium ramulus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0143
-
 naringenin chalcone mutant enzyme Y48F, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
0.0203
-
 naringenin chalcone mutant enzyme H33A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
0.0439
-
 naringenin chalcone mutant enzyme H73A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
0.0567
-
 naringenin chalcone mutant enzyme R125A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
0.0798
-
 naringenin chalcone mutant enzyme H33Q, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
0.1319
-
 naringenin chalcone mutant enzyme H33E, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
0.354
-
 naringenin chalcone wild type enzyme, at pH 6.4, temperature not specified in the publication Eubacterium ramulus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
194000
-
 gel filtration Eubacterium ramulus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2S)-naringenin Eubacterium ramulus
-
 naringenin chalcone
-
 r
naringenin chalcone Eubacterium ramulus
-
 (2S)-naringenin
-
 r

Organism

Organism UniProt Comment Textmining
Eubacterium ramulus V9P0A9
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, butyl-Sepharose column chromatography, and Superdex 200 gel filtration Eubacterium ramulus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-naringenin
-
 Eubacterium ramulus naringenin chalcone
-
 r
naringenin chalcone
-
 Eubacterium ramulus (2S)-naringenin
-
 r

Subunits

Subunits Comment Organism
homohexamer 6 * 32400, calculated from amino acid sequence Eubacterium ramulus

Synonyms

Synonyms Comment Organism
CHI
-
 Eubacterium ramulus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 naringenin chalcone mutant enzyme H33Q, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
0.2
-
 naringenin chalcone mutant enzyme H33E, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
0.6
-
 naringenin chalcone mutant enzyme H33A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
784
-
 naringenin chalcone mutant enzyme Y48F, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
1142
-
 naringenin chalcone wild type enzyme, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
1313
-
 naringenin chalcone mutant enzyme H73A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
1379
-
 naringenin chalcone mutant enzyme R125A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.9
-
 naringenin chalcone mutant enzyme H33Q, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
1.6
-
 naringenin chalcone mutant enzyme H33E, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
29
-
 naringenin chalcone mutant enzyme H33A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
24000
-
 naringenin chalcone mutant enzyme R125A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
28000
-
 naringenin chalcone mutant enzyme H73A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
32000
-
 naringenin chalcone wild type enzyme, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
55000
-
 naringenin chalcone mutant enzyme Y48F, at pH 6.4, temperature not specified in the publication Eubacterium ramulus