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Literature summary for 5.5.1.6 extracted from
- Molecular cloning and function assay of a chalcone isomerase gene (GbCHI) from Ginkgo biloba (2011), Plant Cell Rep., 30, 49-62.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene GbCHI, DNA and amino acid sequence determination and analysis, real-time quantitative PCR expression analysis, recombinant expression of His-tagged CHI in Escherichia coli strain BL21 (DE3) | Ginkgo biloba |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26290 | - |
x * 26290, about, sequence calculation | Ginkgo biloba |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| naringenin chalcone | Ginkgo biloba | i.e. 6'-hydroxychalcone | naringenin | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ginkgo biloba | - |
gene GbCHI | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged CHI from Escherichia coli strain BL21 (DE3) | Ginkgo biloba |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | developmental expression pattern, overview | Ginkgo biloba | - |
| additional information | GbCHI is expressed in a tissue-specific manner | Ginkgo biloba | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| naringenin chalcone | i.e. 6'-hydroxychalcone | Ginkgo biloba | naringenin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 26290, about, sequence calculation | Ginkgo biloba |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CHI | - |
Ginkgo biloba |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Ginkgo biloba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Ginkgo biloba |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Ginkgo biloba | sequence calulation | - |
7.76 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Ginkgo biloba | gene GbCHI is upregulated by UV-B irradiation or treatment with 5-aminolevulinic acid or three plant growth regulator, i.e. ethylene, abscisic acid, and chlormequat, expression analysis, and analysis of the GbCHI promoter region, overview | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | GbCHI belongs to the chalcone superfamily, phylogenetic tree, overview | Ginkgo biloba |
| metabolism | chalcone isomerase is one of the key enzymes in the flavonoid biosynthesis pathway catalyzing the stereospecific isomerization of chalcones into their corresponding (2S)-flavanones | Ginkgo biloba |
| physiological function | CHI as a key gene regulating flavonoid accumulation in ginkgo leaves | Ginkgo biloba |
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