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Literature summary for 5.5.1.5 extracted from

  • Mazur, P.; Henzel, W.J.; Mattoo, S.; Kozarich, J.W.
    3-Carboxy-cis-cis-muconate lactonizing enzyme from Neurospora crassa: an alternate cycloisomerase motif (1994), J. Bacteriol., 176, 1718-1728.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0103
-
3-carboxy-cis,cis-muconate recombinant enzyme expressed in E. coli Neurospora crassa
0.0207
-
3-carboxy-cis,cis-muconate
-
Neurospora crassa
26.2
-
cis,cis-muconate recombinant enzyme expressed in E. coli Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ contains 0.06 M to 0.3 M of Ca2+ per mol of subunit Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
4 * 41000, SDS-PAGE Neurospora crassa
41200
-
4 * 41200, calculation from nucleotide sequence Neurospora crassa
158000
-
gel filtration Neurospora crassa

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Neurospora crassa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
Neurospora crassa

Purification (Commentary)

Purification (Comment) Organism
-
Neurospora crassa

Reaction

Reaction Comment Organism Reaction ID
3-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 3-carboxy-cis,cis-muconate syn-1,2 addition-elimination reaction Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
370
-
-
Neurospora crassa
600
-
recombinant enzyme expressed in Escherichia coli Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-Carboxy-cis,cis-muconate
-
Aspergillus niger 3-Carboxy-5-oxo-2,5-dihydrofuran-2-acetate
-
?
3-Carboxy-cis,cis-muconate r Neurospora crassa 3-Carboxy-5-oxo-2,5-dihydrofuran-2-acetate
-
?
cis,cis-Muconate
-
Neurospora crassa 5-Oxo-2,5-dihydrofuran-2-acetate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 41000, SDS-PAGE Neurospora crassa
tetramer 4 * 41200, calculation from nucleotide sequence Neurospora crassa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
cis,cis-muconate
-
Neurospora crassa
360
-
3-carboxy-cis,cis-muconate
-
Neurospora crassa
382
-
3-carboxy-cis,cis-muconate recombinant enzyme expressed in E. coli Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
broad around Neurospora crassa