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Literature summary for 5.5.1.4 extracted from
- L-myo-inositol-1-phosphate synthase expressed in developing organ: isolation and characterisation of the enzyme from human fetal liver (2012), Appl. Biochem. Biotechnol., 167, 2269-2282.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.15 | - |
2-deoxy-D-glucose 6-phosphate | pH 7, 37°C, Vmax: 280 nM | Homo sapiens |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | moderate activation | Homo sapiens | |
| Cu2+ | inhibition | Homo sapiens | |
| Hg2+ | inhibition | Homo sapiens | |
| K+ | moderate activation | Homo sapiens | |
| NH4+ | strong activation | Homo sapiens | |
| Zn2+ | inhibition | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 170000 | - |
amino acid sequence | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9NPH2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme is purified 51fold using ammonium sulfate fractionation followed by successive chromatography through DEAE cellulose and BioGel A columns | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | fetal liver | Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, BioGel A purified fractions (final purification fraction), 5-7 days | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-D-glucose 6-phosphate | - |
Homo sapiens | 5-deoxy-L-myo-inositol 1-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-myo-Inositol-1-phosphate synthase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | optimal activity | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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