Literature summary for 5.5.1.4 extracted from

Huang, X.; Hernick, M.
A limitation of the continuous spectrophotometric assay for the measurement of myo-inositol-1-phosphate synthase activity (2011), Anal. Biochem., 417, 228-232.

Search for more literature for 5.5.1.4

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics, effects of different divalent metal ions, overview	Arabidopsis thaliana
0.67	0.72	D-glucose 6-phosphate	pH 7.5, 30°C, dependent on the assay method, presence or absence of Mg2+	Arabidopsis thaliana

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	activates	Arabidopsis thaliana
additional information	the activity of MIPS is moderately enhanced by the addition Mg2+ and is not enhanced by other divalent metal ions, such as Zn2+ and Mn2+	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucose 6-phosphate	Arabidopsis thaliana	-	1L-myo-inositol-1-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose 6-phosphate	-	Arabidopsis thaliana	1L-myo-inositol-1-phosphate	-	?
additional information	development of a continuous spectrophotometric method for measuring MIPS activity using a coupled assay that allows the rapid characterization of MIPS in a multiwell plate format, method validation, overview	Arabidopsis thaliana	?	-	?

Synonyms

Synonyms	Comment	Organism
MIPS	-	Arabidopsis thaliana
Myo-inositol-1-phosphate synthase	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0817	-	D-glucose 6-phosphate	pH 7.5, 30°C, periodate assay method, in absence of 1 mM Mg2+	Arabidopsis thaliana
0.102	-	D-glucose 6-phosphate	pH 7.5, 30°C, periodate assay method, in presence of 1 mM Mg2+	Arabidopsis thaliana
0.107	-	D-glucose 6-phosphate	pH 7.5, 30°C, IMP-coupled assay method, in presence of 1 mM Mg2+	Arabidopsis thaliana
0.3	-	D-glucose 6-phosphate	pH 7.5, 30°C, IMP-coupled assay method, in presence of 10 mM Mg2+	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Arabidopsis thaliana

General Information

General Information	Comment	Organism
metabolism	the reaction catalyzed by MIPS is the first step in the biosynthesis of inositol and inositol-containing molecules that serve important roles in both eukaryotes and prokaryotes	Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.12	-	D-glucose 6-phosphate	pH 7.5, 30°C, periodate assay method, in absence of 1 mM Mg2+	Arabidopsis thaliana
0.14	-	D-glucose 6-phosphate	pH 7.5, 30°C, periodate assay method, in presence of 1 mM Mg2+	Arabidopsis thaliana
0.157	-	D-glucose 6-phosphate	pH 7.5, 30°C, IMP-coupled assay method, in presence of 1 mM Mg2+	Arabidopsis thaliana
0.233	-	D-glucose 6-phosphate	pH 7.5, 30°C, IMP-coupled assay method, in presence of 10 mM Mg2+	Arabidopsis thaliana

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Release 2023.1 (January 2023)