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Literature summary for 5.5.1.4 extracted from
- Identification of myo-inositol-3-phosphate synthase isoforms: characterization, expression, and putative role of a 16-kDa gammac isoform (2009), J. Biol. Chem., 284, 9443-9457.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isoform gammac is expressed in Escherichia coli Rosetta (DE3) pLysS cells | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
gammac isoform, SDS-PAGE | Rattus norvegicus |
| 68000 | - |
fully spliced alpha isoform, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | Q6AYK3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate saturation, phenyl-Sepharose column chromatography, and Q-Sepharose column chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| amygdala | - |
Rattus norvegicus | - |
| brain | moderately high expression | Rattus norvegicus | - |
| brain cortex | - |
Rattus norvegicus | - |
| cerebellum | - |
Rattus norvegicus | - |
| heart | low expression | Rattus norvegicus | - |
| hippocampus | - |
Rattus norvegicus | - |
| intestine | isoform gammac predominates in intestine | Rattus norvegicus | - |
| kidney | moderately high expression | Rattus norvegicus | - |
| liver | moderately high expression | Rattus norvegicus | - |
| lung | moderately high expression | Rattus norvegicus | - |
| additional information | almost no expression in skeletal muscle | Rattus norvegicus | - |
| pancreas | - |
Rattus norvegicus | - |
| spleen | low expression, isoform alpha predominates in spleen | Rattus norvegicus | - |
| testis | isoform alpha predominates in testis | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Glucose 6-phosphate | - |
Rattus norvegicus | L-myo-Inositol 1-phosphate | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | x-ray crystallography | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IP synthase | - |
Rattus norvegicus |
| myo-inositol-3-phosphate synthase | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Rattus norvegicus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | isoform gammac negatively modulates alpha isoform activity, possibly by competing for NAD+, when the gammac isoform is preincubated with NAD+, prior to incubation with the alpha isoform, the decrease is quite pronounced with enzyme activity falling to about 63% at the end of 1 h and to about 40% at the end of 3 h | Rattus norvegicus |
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Release 2023.1 (January 2023)
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