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Literature summary for 5.5.1.34 extracted from

  • Lichman, B.R.; Kamileen, M.O.; Titchiner, G.R.; Saalbach, G.; Stevenson, C.E.M.; Lawson, D.M.; O'Connor, S.E.
    Uncoupled activation and cyclization in catmint reductive terpenoid biosynthesis (2019), Nat. Chem. Biol., 15, 71-79 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Nepeta racemosa

Protein Variants

Protein Variants Comment Organism
K171M isoform Neps1, loss of activity Nepeta racemosa
L156S isoform Neps1, loss of activity Nepeta racemosa
N125A isoform Neps1, partial loss of activity Nepeta racemosa
P155S isoform Neps1, partial loss of activity Nepeta racemosa
S198M isoform Neps1, loss of activity Nepeta racemosa
T152N isoform Neps1, loss of activity Nepeta racemosa
T153A isoform Neps1, activity similar to wild-type Nepeta racemosa
T154G isoform Neps1, improved activity Nepeta racemosa
T202A isoform Neps1, loss of activity Nepeta racemosa
V199A isoform Neps1, activity similar to wild-type Nepeta racemosa
Y167F isoform Neps1, loss of activity Nepeta racemosa

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ 0.1 M NaCl used in assay conditions Nepeta racemosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-8-oxocitronellyl enol Nepeta racemosa
-
(+)-cis,trans-nepetalactol
-
?
8-oxocitronellyl enol Nepeta racemosa
-
(+)-cis,trans-nepetalactol
-
?

Organism

Organism UniProt Comment Textmining
Nepeta racemosa
-
-
-
Nepeta racemosa A0A3Q8GL18 catmint
-
Nepeta racemosa A0A3Q8GYY4 catmint
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, HisTrap column chromatography and Superdex 200 gel filtration Nepeta racemosa

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Nepeta racemosa
-
trichome glandular trichome Nepeta racemosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-8-oxocitronellyl enol
-
Nepeta racemosa (+)-cis,trans-nepetalactol
-
?
8-oxocitronellyl enol
-
Nepeta racemosa (+)-cis,trans-nepetalactol
-
?

Synonyms

Synonyms Comment Organism
nepetalactol-related SDR 1
-
Nepeta racemosa
nepetalactol-related short-chain dehydrogenase/reductase 1
-
Nepeta racemosa
nepetalactol-related short-chain dehydrogenase/reductase 2
-
Nepeta racemosa
NEPS1
-
Nepeta racemosa
NEPS2
-
Nepeta racemosa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Nepeta racemosa

General Information

General Information Comment Organism
physiological function isoforms Neps1 and Neps2 catalyze the stereoselective cyclisation of reactive intermediate (S)-8-oxocitronellyl enol into nepetalactol. Subsequent oxidation of nepetalactol by NEPS1 provides nepetalactone, metabolites known for both insect-repellent activity and euphoric effect in cats Nepeta racemosa