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Literature summary for 5.5.1.32 extracted from

  • Newmister, S.A.; Li, S.; Garcia-Borras, M.; Sanders, J.N.; Yang, S.; Lowell, A.N.; Yu, F.; Smith, J.L.; Williams, R.M.; Houk, K.N.; Sherman, D.H.
    Structural basis of the Cope rearrangement and cyclization in hapalindole biogenesis (2018), Nat. Chem. Biol., 14, 345-351.
    View publication on PubMed
Search for more literature for 5.5.1.32

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Fischerella sp. ATCC 43239

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type and mutants W73M/K132M, Y101F, Y101S, F138S, and Y101F/F138S. enzyme is a dimer with two calcium ions per monomer and with the active sites located at the distal ends of the protein dimer Fischerella sp. ATCC 43239

Protein Variants

Protein Variants Comment Organism
D214A mutation of active site residue, complete loss of activity Fischerella sp. ATCC 43239
D214E mutation of active site residue, complete loss of activity Fischerella sp. ATCC 43239
D214N mutation of active site residue, complete loss of activity Fischerella sp. ATCC 43239
F138S substitution to the corresponding serine residue from FimC5, mutant produces a mixture of wild-type major product, hapalindole, and the FimC5 major product, fischerindole Fischerella sp. ATCC 43239
W73M/K132M introduction of methionine resiudes for crystallization Fischerella sp. ATCC 43239
Y101F/F138S substitution to the corresponding residues from FimC5, mutant produces a mixture of wild-type major product, hapalindole, and the FimC5 major product, fischerindole Fischerella sp. ATCC 43239
Y101S mutant shows reduces activity Fischerella sp. ATCC 43239

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ two calcium ions per protein monomer Fischerella sp. ATCC 43239

Organism

Organism UniProt Comment Textmining
Fischerella sp. ATCC 43239 A0A076NBW8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole
-
 Fischerella sp. ATCC 43239 12-epi-hapalindole U
-
 ?

Synonyms

Synonyms Comment Organism
HpiC1
-
 Fischerella sp. ATCC 43239
HpiU5
-
 Fischerella sp. ATCC 43239