Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.5.1.32 extracted from

  • Li, S.; Lowell, A.; Newmister, S.; Yu, F.; Williams, R.; Sherman, D.
    Decoding cyclase-dependent assembly of hapalindole and fischerindole alkaloids (2017), Nat. Chem. Biol., 13, 467-469.
    View publication on PubMed
Search for more literature for 5.5.1.32

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Fischerella muscicola
expressed in Escherichia coli BL21(DE3) cells Fischerella sp. ATCC 43239
expressed in Escherichia coli BL21(DE3) cells Fischerella sp. IL-199-3-1
expressed in Escherichia coli BL21(DE3) cells Fischerella sp. SAG 46.79
expression in Escherichia coli Fischerella ambigua

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole Fischerella ambigua
-
 12-epi-hapalindole U
-
 ?
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole Fischerella sp. SAG 46.79
-
 12-epi-hapalindole U
-
 ?
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole Fischerella sp. IL-199-3-1
-
 12-epi-hapalindole U
-
 ?
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole Fischerella sp. ATCC 43239
-
 12-epi-hapalindole U
-
 ?
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole Fischerella muscicola
-
 12-epi-hapalindole U
-
 ?
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole Fischerella ambigua UTEX 1903
-
 12-epi-hapalindole U
-
 ?
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole Fischerella muscicola UTEX 1829
-
 12-epi-hapalindole U
-
 ?

Organism

Organism UniProt Comment Textmining
Fischerella ambigua A0A076N4W8
-
-
Fischerella ambigua UTEX 1903 A0A076N4W8
-
-
Fischerella muscicola
-
-
-
Fischerella muscicola UTEX 1829
-
-
-
Fischerella sp. ATCC 43239
-
-
-
Fischerella sp. IL-199-3-1
-
-
-
Fischerella sp. SAG 46.79
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Fischerella muscicola
Ni-NTA agarose column chromatography Fischerella sp. ATCC 43239
Ni-NTA agarose column chromatography Fischerella sp. IL-199-3-1
Ni-NTA agarose column chromatography Fischerella sp. SAG 46.79

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole
-
 Fischerella ambigua 12-epi-hapalindole U
-
 ?
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole
-
 Fischerella sp. SAG 46.79 12-epi-hapalindole U
-
 ?
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole
-
 Fischerella sp. IL-199-3-1 12-epi-hapalindole U
-
 ?
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole
-
 Fischerella sp. ATCC 43239 12-epi-hapalindole U
-
 ?
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole
-
 Fischerella muscicola 12-epi-hapalindole U
-
 ?
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole
-
 Fischerella ambigua UTEX 1903 12-epi-hapalindole U
-
 ?
3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole
-
 Fischerella muscicola UTEX 1829 12-epi-hapalindole U
-
 ?

Synonyms

Synonyms Comment Organism
ambU4
-
 Fischerella ambigua
famC1
-
 Fischerella ambigua
famC1
-
 Fischerella sp. SAG 46.79
famC1
-
 Fischerella sp. IL-199-3-1
famC1
-
 Fischerella sp. ATCC 43239
famC1
-
 Fischerella muscicola
FilC1
-
 Fischerella sp. IL-199-3-1
HpiC1
-
 Fischerella sp. ATCC 43239

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Fischerella ambigua

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Fischerella ambigua

General Information

General Information Comment Organism
physiological function enzyme is involved in ring formation and stereochemical control in the biosynthesis of hapalindoles. FamC1 catalyzes formation of 12-epi-hapalindole U Fischerella ambigua