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Literature summary for 5.5.1.25 extracted from

  • Cho, S.; Kim, J.; Lee, S.
    Identification and characterization of 3,6-anhydro-L-galactonate cycloisomerase belonging to theenolase superfamily (2015), Biotechnol. Bioprocess Eng., 20, 462-472 .
No PubMed abstract available
Search for more literature for 5.5.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Postechiella marina

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ about 50% residual activity at 1 mM Postechiella marina
Ca2+ about 15% residual activity at 1 mM Postechiella marina
Cd2+ about 30% residual activity at 1 mM Postechiella marina
Cu2+ about 40% residual activity at 1 mM Postechiella marina
EDTA about 40% residual activity at 1 mM Postechiella marina
Hg2+ about 45% residual activity at 1 mM Postechiella marina
Ni2+ about 55% residual activity at 1 mM Postechiella marina
Zn2+ about 20% residual activity at 1 mM Postechiella marina

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ about 118% activity at 1 mM Postechiella marina
Mg2+ about 115% activity at 1 mM Postechiella marina
additional information not influenced by Mn2+ and Fe2+ Postechiella marina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,6-anhydro-L-galactonate Postechiella marina
-
 2-dehydro-3-deoxy-L-galactonate
-
 ?
3,6-anhydro-L-galactonate Postechiella marina M091
-
 2-dehydro-3-deoxy-L-galactonate
-
 ?

Organism

Organism UniProt Comment Textmining
Postechiella marina A0A0F7C8X0
-
-
Postechiella marina M091 A0A0F7C8X0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Postechiella marina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,6-anhydro-L-galactonate
-
 Postechiella marina 2-dehydro-3-deoxy-L-galactonate
-
 ?
3,6-anhydro-L-galactonate 100% activity Postechiella marina 2-dehydro-3-deoxy-L-galactonate
-
 r
3,6-anhydro-L-galactonate
-
 Postechiella marina M091 2-dehydro-3-deoxy-L-galactonate
-
 ?
3,6-anhydro-L-galactonate 100% activity Postechiella marina M091 2-dehydro-3-deoxy-L-galactonate
-
 r
galactarate 1.8% activity compared to 3,6-anhydro-L-galactonate Postechiella marina ?
-
 ?
galactarate 1.8% activity compared to 3,6-anhydro-L-galactonate Postechiella marina M091 ?
-
 ?
additional information no activity with D-gluconate, D-galactonate, D-glucuronate, D-glucarate, L-galactono-1,4-lactone, D-lactate, L-lactate, D,L-glycerate, D,L-tartrate, or L-malate Postechiella marina ?
-
 ?
additional information no activity with D-gluconate, D-galactonate, D-glucuronate, D-glucarate, L-galactono-1,4-lactone, D-lactate, L-lactate, D,L-glycerate, D,L-tartrate, or L-malate Postechiella marina M091 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Postechiella marina
? x * 40358, calculated from amino acid sequence Postechiella marina

Synonyms

Synonyms Comment Organism
L-AnGA cycloisomerase
-
 Postechiella marina
L-AnGACI
-
 Postechiella marina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Postechiella marina

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 about 30% activity at 10°C, about 75% activity at 20°C, 100% activity at 30°C, about 90% activity at 40°C, about 60% activity at 50°C, about 30% activity at 60°C Postechiella marina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Postechiella marina

pH Range

pH Minimum pH Maximum Comment Organism
5 10 about 50% activity at pH 5.0, about 70% activity at pH 6.0, about 90% activity at pH 7.0, 100% activity at pH 8.0, about 70% activity at pH 9.0, about 40% activity at pH 10.0 Postechiella marina