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Literature summary for 5.5.1.2 extracted from
- The PA4204 gene encodes a periplasmic gluconolactonase (PpgL) which is important for fitness of Pseudomonas aeruginosa (2008), Microbiology, 154, 2979-2990.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | the enzyme is partially sensitive to EDTA | Pseudomonas aeruginosa |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-Trap nickel affinity column chromatography | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-5-oxo-2-tetrahydrofurancarboxylic acid | - |
Pseudomonas aeruginosa | L-alpha-hydroxyglutaric acid | - |
? | |
| 2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate | - |
Pseudomonas aeruginosa | cis-Butadiene-1,2,4-tricarboxylate | - |
? | |
| D-glucono-D-lactone | - |
Pseudomonas aeruginosa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-Carboxy-cis,cis-muconate lactonizing enzyme | - |
Pseudomonas aeruginosa |
| CMLE | - |
Pseudomonas aeruginosa |
| PA4204 protein | - |
Pseudomonas aeruginosa |
| PpgL | - |
Pseudomonas aeruginosa |
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