BRENDA - Enzyme Database
show all sequences of 5.5.1.2

Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2

Halak, S.; Lehtioe, L.; Basta, T.; Buerger, S.; Contzen, M.; Stolz, A.; Goldman, A.; FEBS J. 273, 5169-5182 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Agrobacterium tumefaciens
Crystallization (Commentary)
Crystallization
Organism
recombinant enzyme at 2.2 A resolution. C-terminal domain present in all other members of fumarase II family is missing and enzyme contains an Arg residue as opposed to Trp in the active site. Activation of the carboxylic nucleophile by a hydrophobic environment is not required for lactonization
Agrobacterium tumefaciens
Inhibitors
Inhibitors
Commentary
Organism
Structure
citrate
noncompetitive, localization of binding pocket on the enzyme outside the active site
Agrobacterium tumefaciens
isocitrate
noncompetitive, localization of binding pocket on the enzyme outside the active site
Agrobacterium tumefaciens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.32
-
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
Agrobacterium tumefaciens
11.3
-
3-sulfomuconate
-
Agrobacterium tumefaciens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
x * 37000, SDS-PAGE
Agrobacterium tumefaciens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium tumefaciens
-
-
-
Agrobacterium tumefaciens
Q2HNZ1
-
-
Agrobacterium tumefaciens S2
Q2HNZ1
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Agrobacterium tumefaciens
Reaction
Reaction
Commentary
Organism
2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate = cis,cis-butadiene-1,2,4-tricarboxylate
activation of the carboxylic nucleophile by a hydrophobic environment is not required for lactonization
Agrobacterium tumefaciens
Storage Stability
Storage Stability
Organism
-20°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, completely stable
Agrobacterium tumefaciens
20°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, 50% loss of activity
Agrobacterium tumefaciens
4°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, 36 days, completely stable
Agrobacterium tumefaciens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
679754
Agrobacterium tumefaciens
cis,cis-butadiene-1,2,4-tricarboxylate
-
-
-
?
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
679754
Agrobacterium tumefaciens S2
cis,cis-butadiene-1,2,4-tricarboxylate
-
-
-
?
3-sulfomuconate
-
679754
Agrobacterium tumefaciens
4-sulfomuconolactone + H2O
-
-
-
?
3-sulfomuconate
-
679754
Agrobacterium tumefaciens S2
4-sulfomuconolactone + H2O
-
-
-
?
additional information
no substrate: 3-sulfomuconate
679754
Agrobacterium tumefaciens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 37000, SDS-PAGE
Agrobacterium tumefaciens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1415
-
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
Agrobacterium tumefaciens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Agrobacterium tumefaciens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
7.4
-
isocitrate
-
Agrobacterium tumefaciens
18
-
citrate
-
Agrobacterium tumefaciens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Agrobacterium tumefaciens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
recombinant enzyme at 2.2 A resolution. C-terminal domain present in all other members of fumarase II family is missing and enzyme contains an Arg residue as opposed to Trp in the active site. Activation of the carboxylic nucleophile by a hydrophobic environment is not required for lactonization
Agrobacterium tumefaciens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
citrate
noncompetitive, localization of binding pocket on the enzyme outside the active site
Agrobacterium tumefaciens
isocitrate
noncompetitive, localization of binding pocket on the enzyme outside the active site
Agrobacterium tumefaciens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
7.4
-
isocitrate
-
Agrobacterium tumefaciens
18
-
citrate
-
Agrobacterium tumefaciens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.32
-
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
Agrobacterium tumefaciens
11.3
-
3-sulfomuconate
-
Agrobacterium tumefaciens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
x * 37000, SDS-PAGE
Agrobacterium tumefaciens
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Agrobacterium tumefaciens
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, completely stable
Agrobacterium tumefaciens
20°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, 50% loss of activity
Agrobacterium tumefaciens
4°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, 36 days, completely stable
Agrobacterium tumefaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
679754
Agrobacterium tumefaciens
cis,cis-butadiene-1,2,4-tricarboxylate
-
-
-
?
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
679754
Agrobacterium tumefaciens S2
cis,cis-butadiene-1,2,4-tricarboxylate
-
-
-
?
3-sulfomuconate
-
679754
Agrobacterium tumefaciens
4-sulfomuconolactone + H2O
-
-
-
?
3-sulfomuconate
-
679754
Agrobacterium tumefaciens S2
4-sulfomuconolactone + H2O
-
-
-
?
additional information
no substrate: 3-sulfomuconate
679754
Agrobacterium tumefaciens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 37000, SDS-PAGE
Agrobacterium tumefaciens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1415
-
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
Agrobacterium tumefaciens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Agrobacterium tumefaciens
Other publictions for EC 5.5.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730426
Michielse
Degradation of aromatic compou ...
Fusarium oxysporum
Mol. Plant Pathol.
13
1089-1100
2012
-
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5
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2
2
-
-
-
694040
Tarighi
The PA4204 gene encodes a peri ...
Pseudomonas aeruginosa
Microbiology
154
2979-2990
2008
-
-
1
-
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1
-
1
-
1
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1
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1
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3
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1
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1
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1
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1
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1
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3
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679754
Halak
Structure and function of the ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens S2
FEBS J.
273
5169-5182
2006
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-
1
1
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2
2
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-
1
-
-
8
-
-
1
1
-
-
-
3
6
1
-
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-
1
1
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2
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1
-
1
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2
2
2
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1
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1
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3
6
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
681790
Halak
Characterization of the genes ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens S2, Hydrogenophaga intermedia, Hydrogenophaga intermedia S1
Microbiology
152
3207-3216
2006
-
-
2
-
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-
2
4
-
-
2
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-
5
-
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2
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2
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6
2
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4
2
4
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-
2
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2
-
4
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2
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2
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-
2
-
6
2
-
-
-
4
2
4
-
-
-
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-
-
-
-
661079
Yang
Crystal structure of 3-carboxy ...
Pseudomonas putida
Biochemistry
43
10424-10434
2004
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-
1
1
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1
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3
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1
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2
2
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1
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1
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1
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1
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-
2
2
-
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-
-
-
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-
-
649257
Merckel
3-Carboxy-cis,cis-muconate lac ...
Neurospora crassa
Acta Crystallogr. Sect. D
D58
727-734
2002
-
-
1
1
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-
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2
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1
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653901
Kajander
The structure of Neurospora cr ...
Neurospora crassa
Structure
10
483-492
2002
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1
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2
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3674
Lorite
Characterization of Bradyrhizo ...
Bradyrhizobium japonicum
Biochim. Biophys. Acta
1397
257-261
1998
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1
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1
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33102
Eulberg
Characterization of a protocat ...
Rhodococcus opacus 1CP, Rhodococcus opacus
J. Bacteriol.
180
1072-1081
1998
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2
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8
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4
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3673
Hasson
Characterization of two crysta ...
Pseudomonas putida
Acta Crystallogr. Sect. D
53
352-353
1997
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1
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2
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1
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1
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3672
Glumoff
Crystallization and preliminar ...
Neurospora crassa
Acta Crystallogr. Sect. D
52
221-223
1996
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1
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3
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1
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1
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1
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3671
Wong
-
Evidence for two uptake system ...
Rhizobium leguminosarum
Arch. Microbiol.
156
385-391
1991
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1
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3668
Doten
Cloning and genetic organizati ...
Acinetobacter calcoaceticus
J. Bacteriol.
169
3168-3174
1987
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1
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3669
Chari
-
Absolute stereochemical course ...
Acinetobacter calcoaceticus, Pseudomonas putida
J. Am. Chem. Soc.
109
5514-5519
1987
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3667
Parke
Enzymes of the beta-ketoadipat ...
Agrobacterium rhizogenes, Agrobacterium tumefaciens, Bradyrhizobium sp., Rhizobium leguminosarum, Sinorhizobium fredii, Sinorhizobium meliloti
J. Bacteriol.
165
288-292
1986
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12
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18
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18
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12
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18
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3666
Patel
Relationship among enzymes of ...
Pseudomonas putida
Biochemistry
12
3531-3537
1973
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1
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2
1
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1
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1
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1
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2
1
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1
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2
1
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1
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1
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2
1
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3663
Thatcher
The purification and physical ...
Aspergillus niger
Biochem. J.
127
32P-33P
1972
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1
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1
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1
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1
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1
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1
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1
1
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3664
Thatcher
The kinetic properties of 3-ca ...
Aspergillus niger
Biochem. J.
127
33P-34P
1972
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2
1
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1
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1
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3665
Willetts
Microbial metabolism of alkylb ...
Bacillus sp. (in: Bacteria)
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1972
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2972
Ornston
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1970
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3661
Ornston
The conversion of catechol and ...
Pseudomonas putida
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1966
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