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Literature summary for 5.5.1.2 extracted from
- The conversion of catechol and protocatechuate to beta-ketoadipate by Pseudomonas putida (1966), J. Biol. Chem., 241, 3787-3794.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoCl2 | - |
Pseudomonas putida |  |
| CuCl2 | - |
Pseudomonas putida | |
| NiCl2 | - |
Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.075 | - |
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 190000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pseudomonas putida | enzyme of the protocatechuate pathway | ? | - |
? | |
| additional information | Pseudomonas putida A.312 | enzyme of the protocatechuate pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate | i.e. beta-carboxy-cis,cis-muconate | Pseudomonas putida | cis-Butadiene-1,2,4-tricarboxylate | i.e. gamma-carboxymuconolactone | ? | |
| 2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate | i.e. beta-carboxy-cis,cis-muconate | Pseudomonas putida A.312 | cis-Butadiene-1,2,4-tricarboxylate | i.e. gamma-carboxymuconolactone | ? | |
| additional information | enzyme of the protocatechuate pathway | Pseudomonas putida | ? | - |
? | |
| additional information | enzyme of the protocatechuate pathway | Pseudomonas putida A.312 | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | pH 5.5: about 70% of maximal activity, pH 9.0: about 25% of maximal activity | Pseudomonas putida |
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Release 2023.1 (January 2023)
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