Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli TOPO10 cells | Capsicum annuum |
Protein Variants | Comment | Organism |
---|---|---|
D127A | the mutation drastically reduces the lycopene cyclase activity of CCS up to 90% | Capsicum annuum |
D259A | the mutation drastically reduces the lycopene cyclase activity of CCS up to 90% | Capsicum annuum |
E128A | the mutation drastically reduces the lycopene cyclase activity of CCS up to 90% | Capsicum annuum |
E296A | the mutation reduces the lycopene cyclase activity of CCS | Capsicum annuum |
E332A | the mutation drastically reduces the lycopene cyclase activity of CCS up to 90% | Capsicum annuum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromoplast | - |
Capsicum annuum | 9509 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
1 * 66000, SDS-PAGE | Capsicum annuum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capsicum annuum | Q42435 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, Affigel 501 gel filtration, Mono-P column chromatography, and Sephacryl gel filtration, and Superose 6 gel filtration | Capsicum annuum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | CCS is mainly dedicated to the synthesis of kappa-cyclic carotenoids (capsanthin and capsorubin) but also has lycopene beta-cyclase activity | Capsicum annuum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000, SDS-PAGE | Capsicum annuum |
Synonyms | Comment | Organism |
---|---|---|
capsanthin-capsorubin synthase | - |
Capsicum annuum |
CCS | - |
Capsicum annuum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains one noncovalently bound FAD that is essential for enzyme activity only in the presence of NADPH, which functions as the FAD reductant | Capsicum annuum | |
NADPH | the enzyme contains one noncovalently bound FAD that is essential for enzyme activity only in the presence of NADPH, which functions as the FAD reductant | Capsicum annuum |