Literature summary for 5.5.1.16 extracted from

Zhang, Y.; Prach, L.M.; OBrien, T.E.; DiMaio, F.; Prigozhin, D.M.; Corn, J.E.; Alber, T.; Siegel, J.B.; Tantillo, D.J.
Crystal structure and mechanistic molecular modeling studies of Mycobacterium tuberculosis diterpene cyclase Rv3377c (2020), Biochemistry, 59, 4507-4515.

Search for more literature for 5.5.1.16

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-CodonPlus(DE3) cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 8000 and 0.1 M CHES (pH 9.5) with 2 mM MgCl2	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geranylgeranyl diphosphate	Mycobacterium tuberculosis	-	tuberculosinyl diphosphate	-	?
geranylgeranyl diphosphate	Mycobacterium tuberculosis H37Rv	-	tuberculosinyl diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	O50406	-	-
Mycobacterium tuberculosis H37Rv	O50406	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal affinity chromatography	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranylgeranyl diphosphate	-	Mycobacterium tuberculosis	tuberculosinyl diphosphate	-	?
geranylgeranyl diphosphate	-	Mycobacterium tuberculosis H37Rv	tuberculosinyl diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
HPS	-	Mycobacterium tuberculosis
Rv3377c	-	Mycobacterium tuberculosis

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Release 2026.1 (March 2026)