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Literature summary for 5.5.1.16 extracted from

  • Lemke, C.; Roach, K.; Ortega, T.; Tantillo, D.J.; Siegel, J.B.; Peters, R.J.
    Investigation of acid-base catalysis in halimadienyl diphosphate synthase involved in Mycobacterium tuberculosis virulence (2022), ACS Bio Med Chem Au, 2, 490-498.
    View publication on PubMed
Search for more literature for 5.5.1.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Star cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
A234N/F235V the mutant predominantly produces tuberculosinyl diphosphate, with trace amounts of labda-8,13-dienyl diphosphate and labda-7,13-dienyl diphosphate from geranylgeranyl diphosphate Mycobacterium tuberculosis
D295A almost completely inactive Mycobacterium tuberculosis
F171A the mutant forms small amounts of tuberculosinyl diphosphate and labda-8,13-dienyl diphosphate, along with trace amounts of labda-7,13-dienyl diphosphate from geranylgeranyl diphosphate Mycobacterium tuberculosis
F171H the mutant generates significant amounts of copalyl diphosphate from geranylgeranyl diphosphate Mycobacterium tuberculosis
F171H/A234N/F235V the mutations lead to an almost complete loss of metabolic flux Mycobacterium tuberculosis
Y328F the mutant generates an alternative product (labda-7,13-dienyl diphosphate) from geranylgeranyl diphosphate Mycobacterium tuberculosis
Y328F/Y479F the mutant generates an alternative product (labda-7,13-dienyl diphosphate) from geranylgeranyl diphosphate Mycobacterium tuberculosis
Y479F the mutant generates an alternative product (labda-7,13-dienyl diphosphate) from geranylgeranyl diphosphate Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate Mycobacterium tuberculosis
-
 tuberculosinyl diphosphate
-
 ?
geranylgeranyl diphosphate Mycobacterium tuberculosis H37Rv
-
 tuberculosinyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis O50406
-
-
Mycobacterium tuberculosis H37Rv O50406
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate
-
 Mycobacterium tuberculosis tuberculosinyl diphosphate
-
 ?
geranylgeranyl diphosphate
-
 Mycobacterium tuberculosis H37Rv tuberculosinyl diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
halimadienyl diphosphate synthase
-
 Mycobacterium tuberculosis
HPS
-
 Mycobacterium tuberculosis
Rv3377c
-
 Mycobacterium tuberculosis