Literature summary for 5.5.1.16 extracted from

Mann, F.M.; Prisic, S.; Hu, H.; Xu, M.; Coates, R.M.; Peters, R.J.
Characterization and inhibition of a class II diterpene cyclase from Mycobacterium tuberculosis: Implications for tuberculosis (2009), J. Biol. Chem., 284, 23574-23579.

Search for more literature for 5.5.1.16

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain C41	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
(E,E,E)-geranylgeranyl diphosphate	substrate inhibition	Mycobacterium tuberculosis
15-aza-14,15-dihydrogeranylgeranyl diphosphate	-	Mycobacterium tuberculosis
15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate	-	Mycobacterium tuberculosis
Mg2+	the enzyme displays a rapid loss of activity as the Mg2+ concentration is raised above 0.1 mM	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0016	-	(E,E,E)-geranylgeranyl diphosphate	-	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	the enzyme requires Mg2+ and reacts most efficiently in the presence of low levels (0.1 mM) of Mg2+	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-
Mycobacterium tuberculosis H37Rv	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
amylose resin column chromatography	Mycobacterium tuberculosis

Storage Stability

Storage Stability	Organism
4°C, less than 24 h, the enzyme retains essentially full activity	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(E,E,E)-geranylgeranyl diphosphate	-	Mycobacterium tuberculosis	halimadienyl diphosphate	-	?
(E,E,E)-geranylgeranyl diphosphate	-	Mycobacterium tuberculosis H37Rv	halimadienyl diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
HPS	-	Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
-4	-	freezing leads to loss of about 10% activity over the course of 1 week	Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.12	-	(E,E,E)-geranylgeranyl diphosphate	-	Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.018	-	(E,E,E)-geranylgeranyl diphosphate	-	Mycobacterium tuberculosis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0000001	-	-	Mycobacterium tuberculosis	15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate
0.000002	-	-	Mycobacterium tuberculosis	15-aza-14,15-dihydrogeranylgeranyl diphosphate

General Information

General Information	Comment	Organism
metabolism	class II diterpene cyclase that catalyzes the committed step in edaxadiene biosynthesis	Mycobacterium tuberculosis

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Release 2026.1 (March 2026)