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Literature summary for 5.5.1.16 extracted from

  • Mann, F.M.; Prisic, S.; Hu, H.; Xu, M.; Coates, R.M.; Peters, R.J.
    Characterization and inhibition of a class II diterpene cyclase from Mycobacterium tuberculosis: Implications for tuberculosis (2009), J. Biol. Chem., 284, 23574-23579.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain C41 Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
(E,E,E)-geranylgeranyl diphosphate substrate inhibition Mycobacterium tuberculosis
15-aza-14,15-dihydrogeranylgeranyl diphosphate
-
 Mycobacterium tuberculosis
15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate
-
 Mycobacterium tuberculosis
Mg2+ the enzyme displays a rapid loss of activity as the Mg2+ concentration is raised above 0.1 mM Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 (E,E,E)-geranylgeranyl diphosphate
-
 Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme requires Mg2+ and reacts most efficiently in the presence of low levels (0.1 mM) of Mg2+ Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography Mycobacterium tuberculosis

Storage Stability

Storage Stability Organism
4°C, less than 24 h, the enzyme retains essentially full activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E,E)-geranylgeranyl diphosphate
-
 Mycobacterium tuberculosis halimadienyl diphosphate
-
 ?
(E,E,E)-geranylgeranyl diphosphate
-
 Mycobacterium tuberculosis H37Rv halimadienyl diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
HPS
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-4
-
 freezing leads to loss of about 10% activity over the course of 1 week Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 (E,E,E)-geranylgeranyl diphosphate
-
 Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.018
-
 (E,E,E)-geranylgeranyl diphosphate
-
 Mycobacterium tuberculosis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000001
-
-
 Mycobacterium tuberculosis 15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate
0.000002
-
-
 Mycobacterium tuberculosis 15-aza-14,15-dihydrogeranylgeranyl diphosphate

General Information

General Information Comment Organism
metabolism class II diterpene cyclase that catalyzes the committed step in edaxadiene biosynthesis Mycobacterium tuberculosis