Literature summary for 5.5.1.15 extracted from

Hamano, Y.; Kuzuyama, T.; Itoh, N.; Furihata, K.; Seto, H.; Dairi, T.
Functional analysis of eubacterial diterpene cyclases responsible for biosynthesis of a diterpene antibiotic, terpentecin (2002), J. Biol. Chem., 277, 37098-37104.

Search for more literature for 5.5.1.15

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	20% glycerol, 5 mM 2-mercaptoethanol, and 0.1% Tween 80 are required for the full activity of the Cyc1	Kitasatospora griseola

Cloned(Commentary)

Cloned (Comment)	Organism
-	Kitasatospora griseola

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	-	Kitasatospora griseola
geranylgeranyl diphosphate	above 0.05 mM	Kitasatospora griseola

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0642	-	geranylgeranyl diphosphate	-	Kitasatospora griseola

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	the enzyme activity of the Cyc1 is highest at a concentration of 1 mM but slightly inhibited at a concentration of 10 mM (decreased 40%)	Kitasatospora griseola
additional information	no activity is detected with other divalent metal ions such as Ca2+, Co2+, Cu2+, Fe2+, Mn2+, and Zn2+ at both 1 and 10 mM	Kitasatospora griseola

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	gel filtration	Kitasatospora griseola

Organism

Organism	UniProt	Comment	Textmining
Kitasatospora griseola	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranylgeranyl diphosphate	-	Kitasatospora griseola	terpentedienyl diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
Cyc1	-	Kitasatospora griseola

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	30	-	Kitasatospora griseola

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
25	50	25-30: optimum, 50°C, no activity detected above	Kitasatospora griseola

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	-	full activity after incubation at 30°C in 0.05 M Tris-HCl buffer for 1 h	Kitasatospora griseola

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	-	Tris-HCl buffer	Kitasatospora griseola

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)