Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 5 mM, required for full activity | Streptomyces sp. | |
Tween-80 | 0.1%, required for full activity | Streptomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
geranylgeranyl diphosphate | inhibitory above 0.03 mM | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0137 | - |
geranylgeranyl diphosphate | 35°C, pH 5.5 | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | or Zn2+, Mg2+, required. Activation in decreasing order: Mg2+, Zn2+, Co2+ | Streptomyces sp. | |
Mg2+ | or Zn2+, Co2+, required. Activation in decreasing order: Mg2+, Zn2+, Co2+ | Streptomyces sp. | |
Zn2+ | or Mg2+, Co2+, required. Activation in decreasing order: Mg2+, Zn2+, Co2+ | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Streptomyces sp. KO-3988 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | - |
Streptomyces sp. | ent-copalyl diphosphate | - |
? | |
geranylgeranyl diphosphate | - |
Streptomyces sp. KO-3988 | ent-copalyl diphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
pH 5.5, stable for at least 1 h | Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
geranylgeranyl diphosphate | 35°C, pH 5.5 | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Streptomyces sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | more than 90% of maximum activity within this range | Streptomyces sp. |