Application | Comment | Organism |
---|---|---|
synthesis | the development of yeast strains carrying the engineered Erg20p, which support efficient isoprenoid production, e.g. by abietadiene synthase, and can be used as a dedicated chassis for diterpene production or biosynthetic pathway elucidation. The design developed can be applied to the production of any GGPP-derived isoprenoid and is compatible with other yeast terpene production platforms, method overview | Picea abies |
Cloned (Comment) | Organism |
---|---|
gene TPS-LAS, recombinant expression in Saccharomyces cerevisiae strain AM94, a yeast strain engineered for the production of isoprenoids, which contains three chromosomally integrated copies of a degradation stabilized variant of HMG2 and a heterozygous deletion of ERG9, and in strain AM204, each with or without coexpression of yeast protein Erg20p(F96C), a yeast FPP synthase, overview | Picea abies |
Protein Variants | Comment | Organism |
---|---|---|
additional information | using a combination of protein and genetic engineering, significant improvements in the production of sclareol and several other isoprenoids is achieved, including cis-abienol, abietadiene, and beta-carotene. Recombinant expression of enzyme PaLAS in Saccharomyces cerevisiae AM94 cells, coexpression with different Saccharomyces cerevisiae farnesyl diphosphate synthase ERG20 variants increases diterpene yields by up to 15fold compared to expression of the Cistus creticus GGPPS | Picea abies |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Picea abies |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | Picea abies | - |
(+)-copalyl diphosphate | - |
? | |
additional information | Picea abies | abietadiene synthase (PaLAS) is a bifunctional terpene synthase that catalyzes both class I and class II reactions, i.e. formation of (+)-copalyl diphosphate from geranylgeranyl diphosphate (EC 5.5.1.12, class II diTPS), and formation of abieta-8(14),12-diene from (+)-copalyl diphosphate (cf. EC 4.2.3.32, class I diTPS) | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picea abies | Q675L4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | - |
Picea abies | (+)-copalyl diphosphate | - |
? | |
additional information | abietadiene synthase (PaLAS) is a bifunctional terpene synthase that catalyzes both class I and class II reactions, i.e. formation of (+)-copalyl diphosphate from geranylgeranyl diphosphate (EC 5.5.1.12, class II diTPS), and formation of abieta-8(14),12-diene from (+)-copalyl diphosphate (cf. EC 4.2.3.32, class I diTPS) | Picea abies | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
abietadiene synthase | - |
Picea abies |
More | cf. EC 4.2.3.32 | Picea abies |
PaLAS | - |
Picea abies |
TPS-LAS | - |
Picea abies |
General Information | Comment | Organism |
---|---|---|
evolution | the bifunctional enzyme belongs to the class I and II diterpene synthases | Picea abies |