Literature summary for 5.5.1.12 extracted from

Cui, G.; Duan, L.; Jin, B.; Qian, J.; Xue, Z.; Shen, G.; Snyder, J.H.; Song, J.; Chen, S.; Huang, L.; Peters, R.J.; Qi, X.
Functional divergence of diterpene syntheses in the medicinal plant Salvia miltiorrhiza (2015), Plant Physiol., 169, 1607-1618 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene SmCPS1, phylogenetic analysis, a 289-bp gene-specific sequence including the 3' UTR of SmCPS1 is amplified by PCR using cDNA as a template and then cloned into the pK7GWIWG (II) binary, then introduced into Agrobacterium tumefaciens strain EHA105 by electroporation for ransformation of leaf explants from Salvia miltiorrhiza bh2-7 plants, recombinant expression of His-tagged enzyme in Escherichia coli strains Tuner (DE3) or Origami B (DE3)	Salvia miltiorrhiza f. alba
gene SmCPS2, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strains Tuner (DE3) or Origami B (DE3)	Salvia miltiorrhiza f. alba

Cloned (Comment)

Organism

gene SmCPS1, phylogenetic analysis, a 289-bp gene-specific sequence including the 3' UTR of SmCPS1 is amplified by PCR using cDNA as a template and then cloned into the pK7GWIWG (II) binary, then introduced into Agrobacterium tumefaciens strain EHA105 by electroporation for ransformation of leaf explants from Salvia miltiorrhiza bh2-7 plants, recombinant expression of His-tagged enzyme in Escherichia coli strains Tuner (DE3) or Origami B (DE3)

Salvia miltiorrhiza f. alba

gene SmCPS2, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strains Tuner (DE3) or Origami B (DE3)

Salvia miltiorrhiza f. alba

Protein Variants

Protein Variants	Comment	Organism
additional information	knockdown of SmCPS1 by RNAi	Salvia miltiorrhiza f. alba

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geranylgeranyl diphosphate	Salvia miltiorrhiza f. alba	-	(+)-copalyl diphosphate	-	r
geranylgeranyl diphosphate	Salvia miltiorrhiza f. alba bh2-7	-	(+)-copalyl diphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Salvia miltiorrhiza f. alba	A0A0A7AN31	-	-
Salvia miltiorrhiza f. alba	A0A0A7ANT1	-	-
Salvia miltiorrhiza f. alba bh2-7	A0A0A7AN31	-	-
Salvia miltiorrhiza f. alba bh2-7	A0A0A7ANT1	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme by nickel affinity chromatography from Escherichia coli strains Tuner (DE3) or Origami B (DE3)	Salvia miltiorrhiza f. alba

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranylgeranyl diphosphate	-	Salvia miltiorrhiza f. alba	(+)-copalyl diphosphate	-	r
geranylgeranyl diphosphate	-	Salvia miltiorrhiza f. alba bh2-7	(+)-copalyl diphosphate	-	r

Synonyms

Synonyms	Comment	Organism
CPS1	-	Salvia miltiorrhiza f. alba
CPS2	-	Salvia miltiorrhiza f. alba
SmCPS1	-	Salvia miltiorrhiza f. alba
SmCPS2	-	Salvia miltiorrhiza f. alba

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Salvia miltiorrhiza f. alba

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Salvia miltiorrhiza f. alba

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the diterpene synthase family	Salvia miltiorrhiza f. alba
additional information	metabolomic profiling, and homology modeling and molecular docking	Salvia miltiorrhiza f. alba