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Literature summary for 5.5.1.10 extracted from

  • Dubessay, P.; Larroche, C.; Fontanille, P.
    Cloning and characterization of the gene encoding alpha-pinene oxide lyase enzyme (Pralpha-POL) from Pseudomonas rhodesiae CIP 107491 and production of the recombinant protein in Escherichia coli (2018), Appl. Biochem. Biotechnol., 185, 676-690 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(lambdDE3) cells Pseudomonas rhodesiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-pinene oxide Pseudomonas rhodesiae
-
 (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?
alpha-pinene oxide Pseudomonas rhodesiae CIP 107491
-
 (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas rhodesiae A0A0U2U334
-
-
Pseudomonas rhodesiae CIP 107491 A0A0U2U334
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and Superdex 200 gel filtration Pseudomonas rhodesiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-pinene oxide
-
 Pseudomonas rhodesiae (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?
alpha-pinene oxide
-
 Pseudomonas rhodesiae CIP 107491 (Z)-2-methyl-5-isopropylhexa-2,5-dienal
-
 ?

Subunits

Subunits Comment Organism
? x * 23700, calculated from amino acid sequence Pseudomonas rhodesiae
? x * 25000, SDS-PAGE Pseudomonas rhodesiae

Synonyms

Synonyms Comment Organism
alpha-pinene oxide lyase
-
 Pseudomonas rhodesiae
Pralpha-POL
-
 Pseudomonas rhodesiae

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas rhodesiae calculated from amino acid sequence
-
 5.2