Literature summary for 5.5.1.1 extracted from

Camara, B.; Nikodem, P.; Bielecki, P.; Bobadilla, R.; Junca, H.; Pieper, D.H.
Characterization of a gene cluster involved in 4-chlorocatechol degradation by Pseudomonas reinekei MT1 (2009), J. Bacteriol., 191, 4905-4915.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0106	-	3-methylmuconate	-	Pseudomonas reinekei
0.04	-	2-methylmuconate	-	Pseudomonas reinekei
0.105	-	3-chloromuconate	-	Pseudomonas reinekei

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39760	-	subunit, calculated from amino acid sequence	Pseudomonas reinekei
43000	-	8 * 43000, SDS-PAGE	Pseudomonas reinekei
350000	-	gel filtration	Pseudomonas reinekei

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas reinekei	C6YXG6	-	-

Purification (Commentary)

Purification (Comment)	Organism
MonoQ column chromatography and Superose 12 gel filtration	Pseudomonas reinekei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-methylmuconate	-	Pseudomonas reinekei	?	-	?
3-chloromuconate	the highest turnover rate, 10fold higher than with 3-methylmuconate, is observed with 3-chloromuconate	Pseudomonas reinekei	cis-dienelactone + protoanemonin	-	?
3-methylmuconate	-	Pseudomonas reinekei	?	-	?
additional information	activity of the enzyme with muconate is negligible and at a substrate concentration of 0.1 mM substrate, the activity is only 0.4% of that with 3-chloromuconate, the enzyme is practically inactive with 2-chloromuconate	Pseudomonas reinekei	?	-	?

Subunits

Subunits	Comment	Organism
homooctamer	8 * 43000, SDS-PAGE	Pseudomonas reinekei

Synonyms

Synonyms	Comment	Organism
(chloro)muconate cycloisomerase	-	Pseudomonas reinekei
MCI	-	Pseudomonas reinekei
MCIccaB	a functional intermediate between chloromuconate cycloisomerases and muconate cycloisomerases	Pseudomonas reinekei
muconate cycloisomerase	-	Pseudomonas reinekei

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.5	-	2-methylmuconate	-	Pseudomonas reinekei
11	-	3-methylmuconate	-	Pseudomonas reinekei
111	-	3-chloromuconate	-	Pseudomonas reinekei

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
10	-	2-methylmuconate	-	Pseudomonas reinekei
1000	-	3-methylmuconate	-	Pseudomonas reinekei
1100	-	3-chloromuconate	-	Pseudomonas reinekei

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Release 2023.1 (January 2023)