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Literature summary for 5.5.1.1 extracted from
- Phenol degradation by Rhodococcus opacus strain 1G (2009), Appl. Biochem. Microbiol., 45, 43-49.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0071 | - |
cis,cis-muconate | at 25°C | Rhodococcus opacus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the absence of MnCl2 in the reaction medium has no effect on the activity of MCI | Rhodococcus opacus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus opacus | - |
- |
- |
| Rhodococcus opacus 1G | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose column chromatography | Rhodococcus opacus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.49 | - |
cell-free extract | Rhodococcus opacus |
| 8.3 | - |
after 17fold purification | Rhodococcus opacus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylmuconate | 27% activity compared to cis,cis-muconate | Rhodococcus opacus | ? | - |
? | |
| 2-methylmuconate | 27% activity compared to cis,cis-muconate | Rhodococcus opacus 1G | ? | - |
? | |
| 3-chloromuconate | 9% activity compared to cis,cis-muconate | Rhodococcus opacus | ? | - |
? | |
| 3-chloromuconate | 9% activity compared to cis,cis-muconate | Rhodococcus opacus 1G | ? | - |
? | |
| 3-methylmuconate | 26% activity compared to cis,cis-muconate | Rhodococcus opacus | ? | - |
? | |
| 3-methylmuconate | 26% activity compared to cis,cis-muconate | Rhodococcus opacus 1G | ? | - |
? | |
| cis,cis-muconate | best substrate | Rhodococcus opacus | muconolactone | - |
? | |
| cis,cis-muconate | best substrate | Rhodococcus opacus 1G | muconolactone | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MCI | - |
Rhodococcus opacus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Rhodococcus opacus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
the activity of MCI persists after heating at 60°C for 15 min | Rhodococcus opacus |
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Release 2023.1 (January 2023)
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