KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
cis,cis-muconate | pH 8.0 | Rhodococcus rhodochrous |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
6 * 40000, SDS-PAGE | Rhodococcus rhodochrous |
236000 | - |
gel filtration | Rhodococcus rhodochrous |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus rhodochrous | - |
N75 | - |
Rhodococcus rhodochrous N75 | - |
N75 | - |
Purification (Comment) | Organism |
---|---|
a single cycloisomerase with no isoforms | Rhodococcus rhodochrous |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodococcus rhodochrous |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dimethyl-cis,cis-muconate | - |
Rhodococcus rhodochrous | 2,3-dimethylmuconolactone | - |
? | |
2,4-dimethyl-cis,cis-muconate | - |
Rhodococcus rhodochrous | 2,4-dimethylmuconolactone | - |
? | |
2-methyl-cis,cis-muconate | - |
Rhodococcus rhodochrous | 2-methylmuconolactone | - |
? | |
2-methyl-cis,cis-muconate | - |
Rhodococcus rhodochrous N75 | 2-methylmuconolactone | - |
? | |
3-ethyl-cis,cis-muconate | - |
Rhodococcus rhodochrous | 4-ethylmuconolactone | - |
? | |
3-ethyl-cis,cis-muconate | - |
Rhodococcus rhodochrous N75 | 4-ethylmuconolactone | - |
? | |
3-methyl-cis,cis-muconate | - |
Rhodococcus rhodochrous | 4-methylmuconolactone | - |
? | |
3-methyl-cis,cis-muconate | - |
Rhodococcus rhodochrous N75 | 4-methylmuconolactone | - |
? | |
cis,cis-muconate | - |
Rhodococcus rhodochrous | (4S)-muconolactone | - |
? | |
cis,cis-muconate | - |
Rhodococcus rhodochrous N75 | (4S)-muconolactone | - |
? | |
additional information | the methyl substitution at the C-2 position of muconate directs the cycloisomerization by 1,4-addition rather than 3,6-addition. The steric influence caused by methyl substitution may encourage the 1,4-cycloisomerization | Rhodococcus rhodochrous | ? | - |
? | |
additional information | the methyl substitution at the C-2 position of muconate directs the cycloisomerization by 1,4-addition rather than 3,6-addition. The steric influence caused by methyl substitution may encourage the 1,4-cycloisomerization | Rhodococcus rhodochrous N75 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 40000, SDS-PAGE | Rhodococcus rhodochrous |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rhodococcus rhodochrous |