Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | - |
Rhodococcus erythropolis | |
DTNB | - |
Rhodococcus erythropolis | |
EDTA | - |
Rhodococcus erythropolis | |
HgCl2 | - |
Rhodococcus erythropolis | |
nitriloacetic acid | - |
Rhodococcus erythropolis | |
PCMB | - |
Rhodococcus erythropolis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | is the best metal ion activator | Rhodococcus erythropolis | |
Mn2+ | activates to a lower extent than Co2+ | Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | Rhodococcus erythropolis |
400000 | - |
gel filtration | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis,cis-Muconate | Rhodococcus erythropolis | catalyzes the second step of the beta-ketoadipate pathway | ? | - |
? | |
cis,cis-Muconate | Rhodococcus erythropolis AN-13 | catalyzes the second step of the beta-ketoadipate pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | - |
AN-13 | - |
Rhodococcus erythropolis AN-13 | - |
AN-13 | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodococcus erythropolis |
Storage Stability | Organism |
---|---|
4°C, pH 7.0, enzyme retains more than 70% of the maximum activity after 24 h | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Methyl-cis,cis-muconate | at 6.1% of the activity relative to cis,cis-muconate | Rhodococcus erythropolis | ? | - |
? | |
2-Methyl-cis,cis-muconate | at 6.1% of the activity relative to cis,cis-muconate | Rhodococcus erythropolis AN-13 | ? | - |
? | |
3-Methyl-cis,cis-muconate | at 5.6% of the activity relative to cis,cis-muconate | Rhodococcus erythropolis | beta-Methylmuconolactone | - |
? | |
3-Methyl-cis,cis-muconate | at 5.6% of the activity relative to cis,cis-muconate | Rhodococcus erythropolis AN-13 | beta-Methylmuconolactone | - |
? | |
cis,cis-Muconate | - |
Rhodococcus erythropolis | (+)-Muconolactone | - |
? | |
cis,cis-Muconate | - |
Rhodococcus erythropolis AN-13 | (+)-Muconolactone | - |
? | |
cis,cis-Muconate | catalyzes the second step of the beta-ketoadipate pathway | Rhodococcus erythropolis | ? | - |
? | |
cis,cis-Muconate | catalyzes the second step of the beta-ketoadipate pathway | Rhodococcus erythropolis AN-13 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Rhodococcus erythropolis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, less than 20% loss of activity | Rhodococcus erythropolis |
65 | - |
10 min, enzyme loses most of its activity | Rhodococcus erythropolis |