Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Gar1 | the activity of Cbf5 is enhanced by protein Gar1 (increases Cbf5's affinity for tRNA, and also directly enhances Cbf5's catalytic activity by increasing the kcat of the reaction) | Pyrococcus furiosus | |
Nop10 | the activity of Cbf5 is enhanced by protein Nop10 (increases Cbf5's affinity for tRNA, and also directly enhances Cbf5's catalytic activity by increasing the kcat of the reaction) | Pyrococcus furiosus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2(DE3) cells | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ Sepharose column chromatography and Superdex 75 gel filtration | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
Cbf5 | - |
Pyrococcus furiosus |
pseudouridine synthase | - |
Pyrococcus furiosus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme usually acts in a guide RNA-dependent manner as part of H/ACA small ribonucleoproteins, however, archaeal Cbf5 can also act independently of guide RNA in modifying uridine 55 in tRNA | Pyrococcus furiosus |