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Literature summary for 5.4.99.B4 extracted from

  • Kamalampeta, R.; Kothe, U.
    Archaeal proteins Nop10 and Gar1 increase the catalytic activity of Cbf5 in pseudouridylating tRNA (2012), Sci. Rep., 2, 663.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Gar1 the activity of Cbf5 is enhanced by protein Gar1 (increases Cbf5's affinity for tRNA, and also directly enhances Cbf5's catalytic activity by increasing the kcat of the reaction) Pyrococcus furiosus
Nop10 the activity of Cbf5 is enhanced by protein Nop10 (increases Cbf5's affinity for tRNA, and also directly enhances Cbf5's catalytic activity by increasing the kcat of the reaction) Pyrococcus furiosus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2(DE3) cells Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ Sepharose column chromatography and Superdex 75 gel filtration Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
Cbf5
-
Pyrococcus furiosus
pseudouridine synthase
-
Pyrococcus furiosus

General Information

General Information Comment Organism
physiological function the enzyme usually acts in a guide RNA-dependent manner as part of H/ACA small ribonucleoproteins, however, archaeal Cbf5 can also act independently of guide RNA in modifying uridine 55 in tRNA Pyrococcus furiosus