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Literature summary for 5.4.99.B38 extracted from

  • Muller, S.; Urban, A.; Hecker, A.; Leclerc, F.; Branlant, C.; Motorin, Y.
    Deficiency of the tRNATyr:Psi35-synthase aPus7 in Archaea of the Sulfolobales order might be rescued by the H/ACA sRNA-guided machinery (2009), Nucleic Acids Res., 37, 1308-1322.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.B38

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and A36F mutant enzymes are produced in Escherichia coli as His6-tagged protein fusions Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pre-tRNATyr(GUA) uridine35 Saccharolobus solfataricus Sulfolobus solfataricus pre-tRNATyr(GUA). No pseudouridylation in mutant pre-tRNATyr(GUA) uridine35 with a U to C mutation at position 35. No pseudouridine formation is detected in the heterologous Pyrococcus abyssi tRNATyr(GUA), which is naturally synthesized without intron pre-tRNATyr(GUA) pseudouridine35
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Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
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Purification (Commentary)

Purification (Comment) Organism
wild-type and A36F mutant enzymes produced in Escherichia coli as His6-tagged protein fusions Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNATyr(GUA) uridine35 Sulfolobus solfataricus pre-tRNATyr(GUA). No pseudouridylation in mutant pre-tRNATyr(GUA) uridine35 with a U to C mutation at position 35. No pseudouridine formation is detected in the heterologous Pyrococcus abyssi tRNATyr(GUA), which is naturally synthesized without intron Saccharolobus solfataricus pre-tRNATyr(GUA) pseudouridine35
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Synonyms

Synonyms Comment Organism
aPus7
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 Saccharolobus solfataricus
H/ACA Sso1 sRNP
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 Saccharolobus solfataricus
pre-tRNATyr:PSI35-synthase
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 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
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 assay at Saccharolobus solfataricus