Any feedback?
Literature summary for 5.4.99.B22 extracted from
- Dual nature of pseudouridylation in U2 snRNA Pus1p-dependent and Pus1p-independent activities in yeasts and higher eukaryotes (2017), RNA, 23, 1060-1067 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of gene deletion mutant DELTApus1. which shows a U2 snRNA modification pattern identical to its parental wild-type strain ED666 | Schizosaccharomyces pombe |
| additional information | generation of Pus1 knockout mice | Mus musculus |
| additional information | SNORA71 is expressed in an enzyme knockout mutant yeast strain, but do not restore pseudouridylation of the endogenous yeast U2 snRNA | Xenopus tropicalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Saccharomyces cerevisiae | Pus1p is the only pseudouridine synthase in Saccharomyces cerevisiae that is active on U2 snRNA at position 44. Yeast U2 snRNA is normally pseudouridylated at positions 35, 42, and 44 | ? | - |
? |  |
| additional information | Saccharomyces cerevisiae BY4741 | Pus1p is the only pseudouridine synthase in Saccharomyces cerevisiae that is active on U2 snRNA at position 44. Yeast U2 snRNA is normally pseudouridylated at positions 35, 42, and 44 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | - |
- |
- |
| Drosophila melanogaster | - |
- |
- |
| Drosophila melanogaster yw | - |
- |
- |
| Mus musculus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae BY4741 | - |
- |
- |
| Schizosaccharomyces pombe | - |
- |
- |
| Schizosaccharomyces pombe 972 | - |
- |
- |
| Xenopus tropicalis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pus1p is the only pseudouridine synthase in Saccharomyces cerevisiae that is active on U2 snRNA at position 44. Yeast U2 snRNA is normally pseudouridylated at positions 35, 42, and 44 | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. Besides U2 and U6 snRNAs in Saccharomyces cerevisiae, the enzyme modifies uridines in many different tRNAs. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-?43 (or 44) in yeasts, Drosophila, and vertebrates | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. In vertrbrates, SCARNA8 (also known as U92 scaRNA) is a guide for U2-Psi43 in addition to established targets U2-Psi34/Psi44. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-?43 (or 44) in yeasts, Drosophila, and vertebrates | Xenopus tropicalis | ? | - |
? | |
| additional information | dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. In vertrbrates, SCARNA8 (also known as U92 scaRNA) is a guide for U2-Psi43 in addition to established targets U2-Psi34/Psi44. U2 snRNA is identified as a genuine substrate for mPus1p. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-Psi43 (or 44) in yeasts, Drosophila, and vertebrates | Mus musculus | ? | - |
? | |
| additional information | dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-?43 (or 44) in yeasts, Drosophila, and vertebrates | Drosophila melanogaster | ? | - |
? | |
| additional information | dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-Psi43 (or 44) in yeasts, Drosophila, and vertebrates | Schizosaccharomyces pombe | ? | - |
? | |
| additional information | dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Pus1p from Caenorhabditis elegans has no enzymatic activity on U2 snRNA when expressed in yeast cells. Substrate specificity analysis and comparison of different species | Caenorhabditis elegans | ? | - |
? | |
| additional information | Pus1p is the only pseudouridine synthase in Saccharomyces cerevisiae that is active on U2 snRNA at position 44. Yeast U2 snRNA is normally pseudouridylated at positions 35, 42, and 44 | Saccharomyces cerevisiae BY4741 | ? | - |
? | |
| additional information | dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. Besides U2 and U6 snRNAs in Saccharomyces cerevisiae, the enzyme modifies uridines in many different tRNAs. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-?43 (or 44) in yeasts, Drosophila, and vertebrates | Saccharomyces cerevisiae BY4741 | ? | - |
? | |
| additional information | dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-?43 (or 44) in yeasts, Drosophila, and vertebrates | Drosophila melanogaster yw | ? | - |
? | |
| additional information | dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-Psi43 (or 44) in yeasts, Drosophila, and vertebrates | Schizosaccharomyces pombe 972 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cePus1p | - |
Caenorhabditis elegans |
| dmPus1p | - |
Drosophila melanogaster |
| mPus1p | - |
Mus musculus |
| Pus1p | - |
Drosophila melanogaster |
| Pus1p | - |
Mus musculus |
| Pus1p | - |
Saccharomyces cerevisiae |
| Pus1p | - |
Schizosaccharomyces pombe |
| Pus1p | - |
Caenorhabditis elegans |
| Pus1p | - |
Xenopus tropicalis |
| spPus1p | - |
Schizosaccharomyces pombe |
| xtPus1p | - |
Xenopus tropicalis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | Pus1p is a member of the TruA pseudouridine synthase family | Drosophila melanogaster |
| evolution | Pus1p is a member of the TruA pseudouridine synthase family | Mus musculus |
| evolution | Pus1p is a member of the TruA pseudouridine synthase family | Saccharomyces cerevisiae |
| evolution | Pus1p is a member of the TruA pseudouridine synthase family | Schizosaccharomyces pombe |
| evolution | Pus1p is a member of the TruA pseudouridine synthase family | Caenorhabditis elegans |
| evolution | Pus1p is a member of the TruA pseudouridine synthase family | Xenopus tropicalis |
| malfunction | in mutant worms, the absence of Pus1p activity on tRNAs has no effect on U2 snRNA modification | Caenorhabditis elegans |
| malfunction | U2 snRNAs from Pus1 knockout mice show no changes in their modification patterns when compared to wild-type U2 snRNAs. In Pus1-knockout mice, Pus1p-dependent modification of tRNAs is missing. No differences between wild-type and mutant mice in their U2 snRNA modification patterns | Mus musculus |
| malfunction | U2 snRNAs from pus1DELTA Schizosaccharomyces pombe knockout strain show no changes in their modification patterns when compared to wild-type U2 snRNAs. A novel box H/ACA RNA is found encoded downstream from the RPC10 gene and experimentally verified its guide RNA activity for positioning Psi43 and Psi44 in U2 snRNA | Schizosaccharomyces pombe |
| additional information | screening for other potential guide RNAs for pseudouridylation of position 43 in U2 snRNA, vertebrate box H/ACA RNAs for antisense elements specific to the U2 snRNA branch point recognition region. The SNORA71 5'-terminal pseudouridylation pocket can base pair with U2 snRNA, both positions 43 and 41 can be modified by base-pairing within the same pseudouridylation pocket | Mus musculus |
| additional information | screening for other potential guide RNAs for pseudouridylation of position 43 in U2 snRNA, vertebrate box H/ACA RNAs for antisense elements specific to the U2 snRNA branch point recognition region. The SNORA71 5'-terminal pseudouridylation pocket can base pair with U2 snRNA, both positions 43 and 41 can be modified by base-pairing within the same pseudouridylation pocket | Xenopus tropicalis |
| additional information | U2 snRNA can be modified in Caenorhabditis elegans by a Pus1p-independent mechanism | Caenorhabditis elegans |
| additional information | U2 snRNA can be modified in Schizosaccharomyces pombe by a Pus1p-independent mechanism | Schizosaccharomyces pombe |
| physiological function | in vertebrate SCARNA8, stronger binding in the alternative configuration to position U2-Psi45 might facilitate efficient sliding to position U2-Psi44. Multiple guide RNAs are typically assigned to the most important modified positions. Pseudouridines at the branch point recognition region of U2 snRNA represent such functionally crucial modifications | Mus musculus |
| physiological function | in vertebrate SCARNA8, stronger binding in the alternative configuration to position U2-Psi45 might facilitate efficient sliding to position U2-Psi44. Multiple guide RNAs are typically assigned to the most important modified positions. Pseudouridines at the branch point recognition region of U2 snRNA represent such functionally crucial modifications | Xenopus tropicalis |
| physiological function | multiple guide RNAs are typically assigned to the most important modified positions. Pseudouridines at the branch point recognition region of U2 snRNA represent such functionally crucial modifications | Drosophila melanogaster |
| physiological function | multiple guide RNAs are typically assigned to the most important modified positions. Pseudouridines at the branch point recognition region of U2 snRNA represent such functionally crucial modifications | Saccharomyces cerevisiae |
| physiological function | multiple guide RNAs are typically assigned to the most important modified positions. Pseudouridines at the branch point recognition region of U2 snRNA represent such functionally crucial modifications | Schizosaccharomyces pombe |
| physiological function | multiple guide RNAs are typically assigned to the most important modified positions. Pseudouridines at the branch point recognition region of U2 snRNA represent such functionally crucial modifications | Caenorhabditis elegans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
