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Literature summary for 5.4.99.9 extracted from
- Aspergillus nidulans cell wall composition and function change in response to hosting several Aspergillus fumigatus UDP-galactopyranose mutase activity mutants (2014), PLoS ONE, 9, e85735 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F66A | site-directed mutagenesis, the residue is located at the end of AfUgmA loop III and may control loop III flipping and consequently opening of the mobile loops depending on the redox state of the cofactor. The colony morphology, cell wall composition, hyphal surface adhesion and response to antifungal drugs of the mutant are altered compared to wild-type | Aspergillus fumigatus |
| H63N | site-directed mutagenesis, the residue is part of the flexible loop (loop III) above the si-face of the isoalloxazine ring. The colony morphology, cell wall composition, hyphal surface adhesion and response to antifungal drugs of the mutant are altered compared to wild-type | Aspergillus fumigatus |
| additional information | Construction of an AnugmADELTA enzyme deletion mutant. An Aspergillus fumigatus enzyme AfUgmA mutant with altered enzyme activity is transformed into enzyme-lacking Aspergillus nidulans mutant AnugmDELTA to assess the effect on growth and wall composition in Aspergillus nidulans. The complemented AnugmA::wild-type AfugmA strain has wild-type phenotype. Aspergillus nidulans strains that host mutated AfUgmA constructs with low enzyme activity show increased hyphal surface adhesion, and AnugmAn and AfugmA-mutated Aspergillus nidulans strains have increased alpha-glucan and decreased beta-glucan in their cell walls compared to wild-type and AfugmA-complemented strains | Aspergillus nidulans |
| additional information | the AfUgmA enzyme deletion mutant shows increased sensitivity to antifungal drugs, particularly caspofungin. Reduced beta-glucan content is correlated with increased caspofungin sensitivity | Aspergillus fumigatus |
| R182A | site-directed mutagenesis, the colony morphology, cell wall composition, hyphal surface adhesion and response to antifungal drugs of the mutant are altered compared to wild-type | Aspergillus fumigatus |
| R182K | site-directed mutagenesis, the colony morphology, cell wall composition, hyphal surface adhesion and response to antifungal drugs of the mutant are altered compared to wild-type | Aspergillus fumigatus |
| R327A | site-directed mutagenesis, the colony morphology, cell wall composition, hyphal surface adhesion and response to antifungal drugs of the mutant are altered compared to wild-type | Aspergillus fumigatus |
| R327K | site-directed mutagenesis, the colony morphology, cell wall composition, hyphal surface adhesion and response to antifungal drugs of the mutant are altered compared to wild-type | Aspergillus fumigatus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | GFP-tagged enzyme | Aspergillus fumigatus | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-galactopyranose | Aspergillus fumigatus | - |
UDP-alpha-D-galactofuranose | - |
r |  |
| UDP-alpha-D-galactopyranose | Aspergillus nidulans | - |
UDP-alpha-D-galactofuranose | - |
r | |
| UDP-alpha-D-galactopyranose | Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139 | - |
UDP-alpha-D-galactofuranose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | Q4W1X2 | i.e. Neosartorya fumigata | - |
| Aspergillus nidulans | Q5B8L8 | i.e. Emericella nidulans | - |
| Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139 | Q5B8L8 | i.e. Emericella nidulans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-galactopyranose | - |
Aspergillus fumigatus | UDP-alpha-D-galactofuranose | - |
r | |
| UDP-alpha-D-galactopyranose | - |
Aspergillus nidulans | UDP-alpha-D-galactofuranose | - |
r | |
| UDP-alpha-D-galactopyranose | - |
Aspergillus nidulans FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139 | UDP-alpha-D-galactofuranose | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ANIA_03112 | - |
Aspergillus nidulans |
| glfA | - |
Aspergillus fumigatus |
| UGM | - |
Aspergillus fumigatus |
| UGM | - |
Aspergillus nidulans |
| UgmA | - |
Aspergillus nidulans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Aspergillus fumigatus | |
| FAD | - |
Aspergillus nidulans | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | AfUgmA residues R182 and R327 are important for its function in vivo, with even conservative amino (RK) substitutions producing AnugmADELTA-phenotype strains. Loss of cell wall alpha-D-galactofuranose is associated with increased hyphal surface adhesion. AfUgmA active site mutations do not affect UgmA-GFP cytoplasmic distribution. Mutant phenotypes, overview | Aspergillus fumigatus |
| malfunction | deletion or repression of Aspergillus nidulans gene ugmA (AnugmA), involved in galactofuranose biosynthesis, impairs growth and increases sensitivity to caspofungin, a beta-1,3-glucan synthesis antagonist. Alteration in galactofuranose affects wall glucan composition in Aspergillus nidulans. Wild-type and complemented wild-type hyphal walls have relatively low alpha-glucan content compared to mutant and AnugmADELTA strains. Mutant phenotypes, overview | Aspergillus nidulans |
| additional information | AfUgmA residues R182 and R327 are important for its function in vivo, with even conservative amino (RK) substitutions producing AnugmADELTA phenotype strains | Aspergillus fumigatus |
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