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Literature summary for 5.4.99.8 extracted from

  • Zhao, H.; Tang, Q.; Mo, C.; Bai, L.; Tu, D.; Ma, X.
    Cloning and characterization of squalene synthase and cycloartenol synthase from Siraitia grosvenorii (2017), Acta Pharm. Sin. B, 7, 215-222 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.8

Cloned(Commentary)

Cloned (Comment) Organism
full-length cDNAs of SgCAS is cloned by a rapid amplification of cDNA-ends with polymerase chain reaction approach. Transient expression in Nicotiana benthamiana Siraitia grosvenorii
gene SgCAS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, gene SgCAS belongs to the cycloartenol synthase cluster, quantitative real-time PCR enzyme expression analysis, transcriptome analysis, recombinant expression in Escherichia coli strain BL21(DE3), transient expression of GFP-tagged SgCAS in Nicotiana benthsmiana lower epidermal cells Siraitia grosvenorii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Siraitia grosvenorii 5829
-
cytosol combined in silico prediction and subcellular localization, experiments in tobacco indicate that cycloartenol synthase is likely located in the nucleus or cytosol Siraitia grosvenorii 5829
-
additional information recombinant GFP-tagged enzyme SgCAS is likely located in the nucleus or cytosol, recombinant GFP-tagged SgCAS in Nicotiana benthamiana is located in the nucleus or cytosol Siraitia grosvenorii
-
-
nucleus
-
 Siraitia grosvenorii 5634
-
nucleus combined in silico prediction and subcellular localization, experiments in tobacco indicate that cycloartenol synthase is likely located in the nucleus or cytosol Siraitia grosvenorii 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene Siraitia grosvenorii
-
 cycloartenol
-
 r

Organism

Organism UniProt Comment Textmining
Siraitia grosvenorii K7NBR1
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Siraitia grosvenorii
-
fruit significantly higher levels in fruits than in other tissues Siraitia grosvenorii
-
leaf low activity Siraitia grosvenorii
-
additional information enzyme SgCAS has a significantly higher level in fruits than in other tissues Siraitia grosvenorii
-
root low activity Siraitia grosvenorii
-
seedling
-
 Siraitia grosvenorii
-
stem low activity Siraitia grosvenorii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene
-
 Siraitia grosvenorii cycloartenol
-
 r

Subunits

Subunits Comment Organism
? x * 87500, about, sequence calculation Siraitia grosvenorii
? x * 87500, calculated from sequence Siraitia grosvenorii

Synonyms

Synonyms Comment Organism
CAS
-
 Siraitia grosvenorii
SgCAS
-
 Siraitia grosvenorii

pI Value

Organism Comment pI Value Maximum pI Value
Siraitia grosvenorii sequence calculation
-
 6.14
Siraitia grosvenorii calculated from sequence
-
 6.14

General Information

General Information Comment Organism
evolution determination of evolutionary relationship of squalene synthases (SQSs) or cycloartneol synthases (CASs) from different plants, phylogenetic trees are constructed, overview Siraitia grosvenorii
metabolism cycloartenol synthase (CAS) is a key enzyme in triterpenoid and steroid biosynthesis. Both SgSQS and SgCAS have significantly higher levels in fruits than in other tissues, suggesting that steroids and mogrosides are competitors for the same precursors in fruits Siraitia grosvenorii