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Literature summary for 5.4.99.8 extracted from

  • Dhar, N.; Rana, S.; Razdan, S.; Bhat, W.W.; Hussain, A.; Dhar, R.S.; Vaishnavi, S.; Hamid, A.; Vishwakarma, R.; Lattoo, S.K.
    Cloning and functional characterization of three branch point oxidosqualene cyclases from Withania somnifera (L.) Dunal (2014), J. Biol. Chem., 289, 17249-17267.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.8

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, cloning and expression in Escherichia coli strain D5alpha, functional expression of GST-tagged enzyme in Schizosaccharomyces pombe Withania somnifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85900
-
 x * 85900, sequence calculation, x * 113000, recombinant GST-tagged enzyme, SDS-PAGE Withania somnifera
113000
-
 x * 85900, sequence calculation, x * 113000, recombinant GST-tagged enzyme, SDS-PAGE Withania somnifera

Organism

Organism UniProt Comment Textmining
Withania somnifera D6R1X1
-
-
Withania somnifera WS-Y-08 D6R1X1
-
-

Subunits

Subunits Comment Organism
? x * 85900, sequence calculation, x * 113000, recombinant GST-tagged enzyme, SDS-PAGE Withania somnifera

Synonyms

Synonyms Comment Organism
OSC/CS
-
 Withania somnifera

pI Value

Organism Comment pI Value Maximum pI Value
Withania somnifera sequence calculation
-
 6.39

Expression

Organism Comment Expression
Withania somnifera plant derived and microbe derived elicitors altered WsOSCs expression pattern both at mRNA and protein level which corresponded with the change in the accumulation of three key withanolides namely withanolide A, withanone, and withaferin A, overview additional information

General Information

General Information Comment Organism
evolution the enzyme belongs to the oxidosqualene cyclase OSC superfamily of enzymes Withania somnifera
metabolism the enzyme is involved in biosynthesis of three key withanolides namely withanolide A, withanone, and withaferin A Withania somnifera