Literature summary for 5.4.99.7 extracted from

Ruf, A.; Muller, F.; D'Arcy, B.; Stihle, M.; Kusznir, E.; Handschin, C.; Morand, O.H.; Thoma, R.
The monotopic membrane protein human oxidosqualene cyclase is active as monomer (2004), Biochem. Biophys. Res. Commun., 315, 247-254.

Search for more literature for 5.4.99.7

Application

Application	Comment	Organism
medicine	target for the development of new hypocjolesterolemic drugs	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapor-diffusion method	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Ro 48-8071	IC50: 8.0 nM for the recombinant enzyme, IC50: 6.5 nM for the human liver enzyme	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	monotopic, integral membrane protein	Homo sapiens	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
80000	-	1 * 80000, SDS-PAGE	Homo sapiens
86000	-	equilibrium sedimentation	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-2,3-epoxysqualene	Homo sapiens	formation of lanosterol the first sterol precursor of cholesterol in mammals	lanosterol	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	the protein is neither glycosylated nor modified post-translationally	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Storage Stability

Storage Stability	Organism
-80°C, 10% glycerol, stable for at least 1 year	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-2,3-epoxysqualene	-	Homo sapiens	lanosterol	-	?
(S)-2,3-epoxysqualene	formation of lanosterol the first sterol precursor of cholesterol in mammals	Homo sapiens	lanosterol	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 80000, SDS-PAGE	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0000065	-	IC50: 6.5 nM for the human liver enzyme	Homo sapiens	Ro 48-8071
0.000008	-	IC50: 8.0 nM for the recombinant enzyme	Homo sapiens	Ro 48-8071

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Release 2023.1 (January 2023)