Literature summary for 5.4.99.64 extracted from

Przybylski, D.; Rohwerder, T.; Harms, H.; Yaneva, N.; Mueller, R.H.
Synthesis of the building block 2-hydroxyisobutyrate from fructose and butyrate by Cupriavidus necator H16 (2013), Appl. Microbiol. Biotechnol., 97, 8875-8885.

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Application

Application	Comment	Organism
synthesis	synthesis of 2-hydroxyisobutanoate in Cupriavidus necator H16 expressing mutase genes HcmA and HcmB. Due to the enantiospecificity of the mutase, fructose is a weaker substrate for 2-hydroxyisobutanoate synthesis than butanoate. Production rates achieved with the poly-3-hydroxybutanoate-negative strain H16 PHB?4 on butanoate are higher than on fructose. Using the wild-type does not significantly improve the production rates, the latter shows a 34fold and a 5fold lower 2-hydroxyisobutanoate synthesis rate compared to H16 PHB?4 on fructose and butanoate, respectively. Both strains show concomitant excretion of undesired side products, such as pyruvate and 3-hydroxybutanoate	Aquincola tertiaricarbonis

Organism

Organism	UniProt	Comment	Textmining
Aquincola tertiaricarbonis	I3VE77 and I3VE74	I3VE77 i.e. large subunit HcmA, I3VE74 i.e. small subunit HcmB	-
Aquincola tertiaricarbonis L108	I3VE77 and I3VE74	I3VE77 i.e. large subunit HcmA, I3VE74 i.e. small subunit HcmB	-

Synonyms

Synonyms	Comment	Organism
HcmA	-	Aquincola tertiaricarbonis
HcmB	-	Aquincola tertiaricarbonis

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Release 2023.1 (January 2023)